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AB113860

Recombinant Human GMDS protein

Recombinant Human GMDS protein

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Recombinant Human GMDS protein is a Human Full Length protein, in the 1 to 372 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

GDP-D-mannose dehydratase, GMD, GMDS

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SDS-PAGE - Recombinant Human GMDS protein (AB113860)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GMDS protein (AB113860)

15% SDS-PAGE analysis of ab113860 (3μg)

Key facts

精製度

>95% SDS-PAGE

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

Mass Spec, SDS-PAGE

applications

生物活性

No

アクセッション番号

O60547

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAHAPARCPSARGSGDGEMGKPRNVALITGITGQDGSYLAEFLLEKGYEVHGIVRRSSSFNTGRIEHLYKNPQAHIEGNMKLHYGDLTDSTCLVKIINEVKPTEIYNLGAQSHVKISFDLAEYTADVDGVGTLRLLDAVKTCGLINSVKFYQASTSELYGKVQEIPQKETTPFYPRSPYGAAKLYAYWIVVNFREAYNLFAVNGILFNHESPRRGANFVTRKISRSVAKIYLGQLECFSLGNLDAKRDWGHAKDYVEAMWLMLQNDEPEDFVIATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKETGKVHVTVDLKYYRPTEVDFLQGDCTKAKQKLNWKPRVAFDELVREMVHADVELMRTNPNA","proteinLength":"Full Length","predictedMolecularWeight":"44.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":372,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O60547","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The GMDS protein also known as GDP-mannose 46-dehydratase plays an important role in catalyzing the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose one step in the biosynthesis of GDP-fucose. It has an approximate mass of 39 kDa and predominantly expresses in the liver kidneys and certain immune cells. The GMDS enzyme operates in the cytosol contributing to cellular metabolism and energy balance providing substrates necessary for further enzymatic conversions.
Biological function summary

GMDS contributes significantly to the fucosylation process a modification critical for the functioning of various glycoproteins and glycolipids. It participates in the synthesis of fucose which serves as a substrate for fucosyltransferases in glycan decorations. The enzymatic activity of GMDS occurs as part of a greater network within the cytosol although it does not directly involve multi-protein complexes. Its product GDP-fucose plays a role in cell-cell interaction and communication emphasizing GMDS’s importance in cell biology and molecular signaling.

Pathways

GMDS contributes to the fucose biosynthesis pathway and indirectly affects the Leloir pathway of galactose metabolism due to interconnected metabolic pools. Through its product GDP-fucose GMDS links to proteins such as fucosyltransferases which incorporate fucose into glycan structures. The availability of fucose also affects signaling pathways involving Notch receptor activation influencing cellular differentiation and growth making GMDS an essential player in glycan-related signal transduction.

GMDS alterations have connections with immunodeficiencies and various cancer types. Deficiencies in GMDS can lead to Congenital Disorder of Glycosylation Type Iic where improper glycosylation affects immune cell functions and fucosylation imbalance links to increased tumor progression and metastasis through altered cell surface glycan structures. In the context of these disorders GMDS influences proteins such as alpha-13-fucosyltransferases with fucose content affecting functional glycan patterns on cells demonstrating its importance in pathophysiology related to glycosylation disorders.
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製品の性状

製品の状態

Liquid

補足情報

ab113860 was purified using conventional chromatography.

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一般的な情報

機能

Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.

配列の類似性

Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.

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製品プロトコール

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ターゲットの情報

Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.
See full target information GDP-mannose 4,6 dehydratase
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組合せ製品

Primary Antibodies

AB97630

Anti-GMDS antibody

Western blot - Anti-GMDS antibody (AB97630)

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