AB276486

Recombinant Human GILT protein (His tag)

Name: Recombinant Human GILT protein (His tag) (ab276486) | Abcam
Brand: Abcam Limited
SKU: AB276486
Price: 75000 JPY
Availability: InStock

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Recombinant Human GILT protein (His tag) is a Human Fragment protein, in the 1 to 243 aa range, expressed in HEK 293 cells, with >96%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

別名を表示する

GILT, IP30, IFI30, Gamma-interferon-inducible lysosomal thiol reductase, Gamma-interferon-inducible protein IP-30, Legumaturain

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SDS-PAGE - Recombinant Human GILT protein (His tag) (AB276486)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GILT protein (His tag) (AB276486)

SDS-PAGE analysis of ab276486

Key facts

精製度

>96% SDS-PAGE

エンドトキシンレベル

< 1 EU/µg

発現系

HEK 293 cells

アプリケーション

SDS-PAGE

applications

生物活性

アクセッション番号

P13284

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.4 Constituents: 100% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"MTLSPLLLFLPPLLLLLDVPTAAVQASPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSS","proteinLength":"Fragment","predictedMolecularWeight":"24.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":243,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P13284","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Gamma-interferon-inducible lysosomal thiol reductase often called GILT is a thiol reductase enzyme with a mass of about 35 kDa. GILT is expressed predominantly in antigen-presenting cells where it resides in the lysosomes. Mechanically GILT catalyzes the reduction of disulfide bonds in proteins which enables proteins to unfold under mild acidic conditions found in lysosomal compartments. GILT activity requires the catalytic cysteine residue which is essential for its function as a reductase.

Biological function summary

GILT contributes to the processing and presentation of antigens. By reducing disulfide bonds in endocytosed proteins GILT prepares them for subsequent degradation by lysosomal proteases. This function means it plays a significant role in the immune response as it aids the generation of peptides presented on major histocompatibility complex (MHC) class II molecules. GILT is not known to be part of a larger protein complex but interacts with other components within the lysosome to accomplish its task.

Pathways

GILT is integral to the antigen processing and presentation pathway mediated by MHC class II molecules. This pathway is critical for the initiation of immune responses and involves collaboration with other proteins such as cathepsins. The reduction of disulfide bonds by GILT allows cathepsins to efficiently degrade proteins into peptide fragments. These peptides then bind to MHC class II molecules which are presented on the cell surface for recognition by CD4+ T cells.

Altered GILT function has been linked to autoimmune diseases and cancer. In certain autoimmune disorders dysregulation of GILT can lead to improper antigen presentation contributing to the body's immune response against its own tissues. In cancer some tumors may downregulate GILT to escape immune surveillance by reducing antigen presentation. Interactions between GILT and other proteins such as MHC class II facilitate these pathological processes highlighting the importance of GILT in maintaining immune system balance.

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity).

配列の類似性

Belongs to the GILT family.

翻訳後修飾

N-glycosylated. Sugar chains contain mannose-6-phosphate.. Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.

細胞内局在性

Lysosome

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information IFI30

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Abcam product promise

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Recombinant Human GILT protein (His tag)