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Recombinant Human Giantin protein (ab152432)

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SDS-PAGE - Recombinant Human Giantin protein (ab152432)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    製品の詳細

    • 製品名

      Recombinant Human Giantin protein

    • 発現系

      Wheat germ

    • アクセッション番号

      Q14789

    • タンパク質長

      Protein fragment

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        SRLSGLANVVLHELSGDDDTDQNMRAPLDPELHQESDMEFNNTTQEDVQE RLAYAEQLVVELKDIIRQKDVQLQQKDEALQEERKAADN

      • 予測される分子量

        36 kDa including tags

      • 領域

        3 to 91

    特性

    Our Abpromise guarantee covers the use of ab152432 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      ELISA

      SDS-PAGE

      Western blot

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • 372 kDa Golgi complex associated protein
      • 372 kDa Golgi complex-associated protein
      • GCP
      • GCP372
      • Giantin
      • GOGB1_HUMAN
      • GOLGB1
      • Golgi autoantigen golgin subfamily a member 1
      • Golgi autoantigen golgin subfamily b macrogolgin (with transmembrane signal) 1
      • Golgi autoantigen, golgin subfamily b, macrogolgin 1
      • Golgi complex associated protein 372kDa
      • Golgi complex-associated protein 372kDa
      • Golgin B1
      • Golgin B1, golgi integral membrane protein
      • Golgin subfamily B member 1
      • GOLIM1
      • Macrogolgin
      • MGC33154
      see all

    • 機能

      May participate in forming intercisternal cross-bridges of the Golgi complex.

    • 細胞内局在

      Golgi apparatus membrane.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human Giantin protein (ab152432)
      SDS-PAGE - Recombinant Human Giantin protein (ab152432)
      12.5% SDS-PAGE analysis of ab152432 stained with Coomassie Blue.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab152432 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab152432 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Giantin-GST (ab152432) was used as used as a bait for the GST-immunoprecipitation of the complexes from HeLa cells lysates

     Excellent
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    To detect the complexes between N-terminal of giantin and other Golgi proteins, we employed the recombinant human N-terminal GST-giantin fusion peptide (3-92 aa). This peptide is successfully immobilized by anti-GST Ab ab19256 coupled Epoxy beads (Dynabeads M-450), according to the GST Western blot (W-B) - see picture.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    投稿 Nov 19 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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