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AB153064

Recombinant Human Gephyrin protein (GST tag N-Terminus)

Recombinant Human Gephyrin protein (GST tag N-Terminus)

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Recombinant Human Gephyrin protein (GST tag N-Terminus) is a Human Fragment protein, in the 677 to 769 aa range, expressed in Wheat germ, suitable for ELISA, WB.

別名を表示する

GPH, KIAA1385, GPHN, Gephyrin

1 Images
SDS-PAGE - Recombinant Human Gephyrin protein (GST tag N-Terminus) (AB153064)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Gephyrin protein (GST tag N-Terminus) (AB153064)

ab153064 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

発現系

Wheat germ

タグ

GST tag N-Terminus

アプリケーション

ELISA, WB

applications

生物活性

No

アクセッション番号

Q9NQX3

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"RKMQGILDPRPTIIKARLSCDVKLDPRPEYHRCILTWHHQEPLPWAQSTGNQMSSRLMSMRSANGLLMLPPKTEQYVELHKGEVVDVMVIGRL","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":769,"aminoAcidStart":677,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9NQX3","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Gephyrin also known as GPHN is a multifunctional protein integral to synaptic transmission. Weighing approximately 93 kDa it facilitates the anchoring of inhibitory neurotransmitter receptors at postsynaptic sites. This protein is expressed primarily in central nervous system tissues including the brain and spinal cord. Gephyrin performs its mechanical role by forming a scaffold to maintain GABAergic and glycinergic receptors' proper positioning and function.
Biological function summary

Gephyrin's function extends beyond receptor clustering. It plays a part in the larger postsynaptic density complex. This scaffolding capability allows gephyrin to organize inhibitory synapses critical for maintaining neuronal excitability and plasticity. The protein contributes by tethering receptors to the cytoskeleton which stabilizes synaptic structures and allows dynamic interactions with other proteins in the synapse.

Pathways

Gephyrin interacts with the inhibitory signaling pathway. This pathway includes important proteins like GABA receptors and glycine receptors which mediate inhibitory synaptic transmission in the central nervous system. Gephyrin's interactions facilitate the formation of gephyrin clusters which influence synaptic inhibition by crosslinking with tubulin and microtubules significantly impacting neuronal signaling.

Disruptions in gephyrin function link to neurological conditions such as epilepsy and schizophrenia. Mutations or alterations in gephyrin expression affect receptor clustering which impacts synapse formation and can result in dysfunctional neural networks. In schizophrenia gephyrin associates with other synaptic proteins such as neuroligin and neurexins indicating potential contributions to synaptic abnormalities seen in the disorder.
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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (PubMed : 25025157, PubMed : 26613940).. Also has a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.

配列の類似性

In the N-terminal section; belongs to the MoaB/Mog family.. In the C-terminal section; belongs to the MoeA family.

翻訳後修飾

Palmitoylated (PubMed:25025157). Palmitoylation is stimulated by GABA type A receptors activity (By similarity). Palmitoylation by ZDHHC12 regulates clustering at synapses (PubMed:25025157).

細胞内局在性

Cytoskeleton

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製品プロトコール

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ターゲットの情報

Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (PubMed : 25025157, PubMed : 26613940).. Also has a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
See full target information GPHN
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組合せ製品

Primary Antibodies

AB177154

Anti-Gephyrin antibody [EPR12651(B)]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Gephyrin antibody [EPR12651(B)] (AB177154)

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