Recombinant human GDNF protein (Active) (ab259417)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Functional Studies, Cell Culture
製品の詳細
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製品名
Recombinant human GDNF protein (Active)
GDNF タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent increase in proliferation of SHSY-5Y cells in the presence of GFRα.
ED50 is ≤9.55 ng/ml, coresponding to a specific activity of 1.05 x 105 units/mg.
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精製度
>= 95 % SDS-PAGE.
=95% by HPLC -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
KRSPDKQMAV LPRRERNRQA AAANPENSRG KGRRGQRGKN RGCVLTAIHL NVTDLGLGYE TKEELIFRYC SGSCDAAETT YDKILKNLSR NRRLVSDKVG QACCRPIAFD DDLSFLDDNL VYHILRKHSA KRCGCI -
予測される分子量
15 kDa -
実際の分子量
15 kDa -
分子量情報
Predicted mass is 15409.58 Da (+/- 10Da by ESI TOF). Observed mass is 15411 Da. -
領域
76 to 211 -
配列の追加情報
N-terminal glycine tag. Full-length mature chain lacking the signal peptide
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特性
Our Abpromise guarantee covers the use of ab259417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
Mass Spectrometry
Functional Studies
Cell Culture
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 6.00
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilised fromThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- Astrocyte derived trophic factor
- Astrocyte derived trophic factor 1
- Astrocyte-derived trophic factor
see all -
機能
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. -
組織特異性
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. -
関連疾患
Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. -
配列類似性
Belongs to the TGF-beta family. GDNF subfamily. -
細胞内局在
Secreted. - Information by UniProt
画像
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Functional Studies - Recombinant human GDNF protein (Active) (ab259417)
Fully biologically active determined by the dose dependent increase in proliferation of SHSY-5Y cells in the presence of GFRα.
ED50 is ≤9.55 ng/ml, coresponding to a specific activity of 1.05 x 105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3368300-1
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SDS-PAGE - Recombinant human GDNF protein (Active) (ab259417)SDS-PAGE analysis of ab259417.
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Mass Spectrometry - Recombinant human GDNF protein (Active) (ab259417)Mass determination by ESI TOF.
Predicted mass is 15409.58 +/- 10Da by ESI TOF. Observed mass is 15411 Da.
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HPLC - Recombinant human GDNF protein (Active) (ab259417)HPLC analysis of ab259417.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab259417 は論文での使用が確認できていません。
