AB259417

Recombinant human GDNF protein (Active)

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Recombinant human GDNF protein (Active) is a Human Full Length protein in the 76 to 211 aa range with ≥95% purity, ≤ 0.005 EU/µg endotoxin level and suitable for Cell Culture, Functional studies, mass spectrometry and SDS-PAGE. The predicted molecular weight of ab259417 protein is 15.4 kDa.

- Save time and ensure accurate results - use our recombinant human GDNF protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs

別名を表示する

Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

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Mass Spectrometry - Recombinant human GDNF protein (Active) (AB259417)

Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human GDNF protein (Active) (AB259417)

Mass determination by ESI TOF.

Predicted mass is 15409.58 +/- 10Da by ESI TOF. Observed mass is 15411 Da.

Functional Studies - Recombinant human GDNF protein (Active) (AB259417)

FuncS

Supplier Data

Functional Studies - Recombinant human GDNF protein (Active) (AB259417)

Fully biologically active determined by the dose dependent increase in proliferation of SHSY-5Y cells in the presence of GFRα.

ED₅₀ is ≤9.55 ng/ml, coresponding to a specific activity of 1.05 x 10⁵ units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3368300-1

HPLC - Recombinant human GDNF protein (Active) (AB259417)

HPLC

Supplier Data

HPLC - Recombinant human GDNF protein (Active) (AB259417)

HPLC analysis of ab259417.

SDS-PAGE - Recombinant human GDNF protein (Active) (AB259417)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GDNF protein (Active) (AB259417)

SDS-PAGE analysis of ab259417.

Key facts

精製度

>95% SDS-PAGE

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

アプリケーション

HPLC, Mass Spec, SDS-PAGE, Cell Culture, FuncS

applications

生物活性

Yes

生物学的活性

Fully biologically active determined by the dose dependent increase in proliferation of SHSY-5Y cells in the presence of GFRα.

ED₅₀ is ≤9.55 ng/ml, coresponding to a specific activity of 1.05 x 10⁵ units/mg.

アクセッション番号

P39905

アニマルフリー

Yes

キャリアフリー

種

Human

再構成

Reconstitute in PBS

バッファー組成

pH: 6 - 8 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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製品の詳細

Ensure the validity of your result using our recombinant human GDNF protein ab259417 as a control.

The ab259417 GDNF protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC.

Check out our protein gel staining guide for SDS-PAGE here

Premium Bioactive Protein range

The ab259417 GDNF protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.

More premium bioactive proteins can be found here

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配列情報

[{"sequence":"KRSPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI","proteinLength":"Full Length","predictedMolecularWeight":"15.4 kDa","actualMolecularWeight":"15.41 kDa","aminoAcidEnd":211,"aminoAcidStart":76,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P39905","tags":[]}]

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

Ambient

長期保存温度

Ambient

True

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製品の性状

製品の状態

Lyophilized

補足情報

=95% by HPLC

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一般的な情報

機能

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed : 8493557). Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor (PubMed : 10829012, PubMed : 25242331, PubMed : 31535977). Involved in the development of the neural crest (PubMed : 15242795).

配列の類似性

Belongs to the TGF-beta family. GDNF subfamily.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information GDNF

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Abcam product promise

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Recombinant human GDNF protein (Active)