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Recombinant human GDF8 / Myostatin protein (ab50097)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: Inhibition Assay, SDS-PAGE, Functional Studies

製品の詳細

  • 製品名

    Recombinant human GDF8 / Myostatin protein
    GDF8 / Myostatin タンパク質・ペプチド 製品一覧

  • 生理活性

    Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected ED50 for this effect is 17.0-25.0 ng/ml.

  • 精製度

    > 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE and HPLC analyses.

  • 発現系

    Escherichia coli

  • アクセッション番号

    O14793

  • タンパク質長

    Protein fragment

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS

    • 予測される分子量

      25 kDa

    • 領域

      267 to 375

特性

Our Abpromise guarantee covers the use of ab50097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Inhibition Assay

    SDS-PAGE

    Functional Studies

  • 製品の状態

    Lyophilized
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

関連情報

  • 別名

    • GDF 8
    • GDF-8
    • GDF8
    • GDF8_HUMAN
    • Growth differentiation factor 8
    • Growth/differentiation factor 8
    • MSLHP
    • MSTN
    • Myostatin
    • OTTHUMP00000163498
    see all

  • 機能

    Acts specifically as a negative regulator of skeletal muscle growth.

  • 配列類似性

    Belongs to the TGF-beta family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab50097 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab50097 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Hi,



I would like to purchase myostatin for a functional study in cells. I saw you have GDF8 / Myostatin protein (Active) ab73251 and https://www.abcam.com/gdf8-myostatin-protein-ab50097.html. What is the difference between these two product. Which one work better in terms of inhibiting MPC-11 cell growth.

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Answer

Thank you for contacting us.

These two proteins are the same, except for the reconstitution instructions (water versus 20 mM Hcl). Both should work equally well in inhiibiting MPC-11 cell growth.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Please provide a protocol for the inhibition assay this antibody has been tested in.

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Answer

Thank you for your recent telephone enquiry. I am pleased to confirm I have obtained the original testing procedure for inhibition assays using ab50097 GDF8 / Myostatin protein. Cytotoxicity Assay for h-Myostatin using MPC-11 cells Procedure: 1. Resuspend cells in assay media containing 10% Horse serum. Transfer 100ul/well to assay plate (10,000 cells/well; passage #7) 2. Serial dilute h-Myostatin in assay media in separate plate in duplicate. 3. Transfer 100ul/well to cells in assay plate 4. Final assay volume of each well is 200ul containing 10% Horse Serum and h-Myostatin as indicated below. 5. Incubate 66 hours 6. Add 20ul of commercially available cell proliferation assay reagent to each well. 7. Incubate 37oC and read OD at 490nm. I hope this is helpful to you. Should you have any further questions, please do not hesitate to contact us again.

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