Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, HPLC
製品名Recombinant human GDF6 protein (Active)
GDF6 タンパク質・ペプチド 製品一覧
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 2.0-3.0 µg/ml.
精製度>= 95 % SDS-PAGE.
= 95% by HPLC..
タンパク質長Full length protein
配列TAFASRHGKRHGKKSRLRCSKKPLHVNFKELGWDDWIIAPLEYEAYHCEG VCDFPLRSHLEPTNHAIIQTLMNSMDPGSTPPSCCVPTKLTPISILYIDA GNNVVYKQYEDMVVESCGCR
領域336 to 455
配列の追加情報Full length chain without signal peptide, without propeptide. Product is 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acid polypeptide chains
Our Abpromise guarantee covers the use of ab245811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Manufactured using all animal-free reagents.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.1% Trifluoroacetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成Reconstitute in water to 0.1-1.0 mg/ml.
- bmp 13
機能Growth factor that controls proliferation and cellular differentiation in the retina and bone formation. Plays a key role in regulating apoptosis during retinal development. Establishes dorsal-ventral positional information in the retina and controls the formation of the retinotectal map (PubMed:23307924). Required for normal formation of bones and joints in the limbs, skull, digits and axial skeleton. Plays a key role in establishing boundaries between skeletal elements during development. Regulation of GDF6 expression seems to be a mechanism for evolving species-specific changes in skeletal strucutres. Seems to positively regulates differentiation of chondrogenic tissue through the growth factor receptors subunits BMPR1A, BMPR1B, BMPR2 and ACVR2A, leading to the activation of SMAD1-SMAD5-SMAD8 complex. The regulation of chondrogenic differentiation is inhibited by NOG (PubMed:26643732). Also involved in the induction of adipogenesis from mesenchymal stem cells. This mechanism acts through the growth factor receptors subunits BMPR1A, BMPR2 and ACVR2A and the activation of SMAD1-SMAD5-SMAD8 complex and MAPK14/p38.
関連疾患Klippel-Feil syndrome 1, autosomal dominant
A chromosomal aberration involving GDF6 has been found in a patient with Klippel-Feil syndrome (KFS). Paracentric inv(8)(q22;2q23.3).
Microphthalmia, isolated, 4
Leber congenital amaurosis 17
Defects in POP1 may be the cause of multiple synostoses syndrome (SYNS). SYNS is a bone disease characterized by multiple progressive joint fusions that commonly involve proximal interphalangeal, tarsal-carpal joints. Additional features can include progressive conductive deafness.
配列類似性Belongs to the TGF-beta family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.