Recombinant human GDF15 protein (ab50077)
Key features and details
- Expression system: CHO cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant human GDF15 protein
GDF15 タンパク質・ペプチド 製品一覧 -
生理活性
Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 osetoblast cells. The expected ED50 for this effect is 75-200 ng/ml.
-
精製度
> 95 % SDS-PAGE.
ab50077 purity was determined also by HPLC -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
CHO cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
ARNGDHCPLGPGRCCRLHTVRASLEDLGWADWVLSPREVQVTMCIGACPS QFRAANMHAQIKTSLHRLKPDTVPAPCCVPASYNPMVLIQKTDTGVSLQT YDDLLAKDCHCI -
予測される分子量
25 kDa -
実際の分子量
25 kDa -
領域
197 to 308 -
配列の追加情報
This product refers to the processed mature form of this protein from aa 195 to 308. Therefore it does not contain the signal peptide or propeptide.
-
特性
Our Abpromise guarantee covers the use of ab50077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
-
製品の状態
Lyophilized -
備考
Molecular Weight: 24.6 kDa
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成Reconstitute to 1mg/ml with distilled water.
関連情報
-
別名
- GDF 15
- GDF-15
- Gdf15
see all -
組織特異性
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab50077 は 3 報の論文で使用されています。
- Radwanska A et al. Increased expression and accumulation of GDF15 in IPF extracellular matrix contribute to fibrosis. JCI Insight 7:N/A (2022). PubMed: 35993367
- Shao M et al. Artemisinin analog SM934 alleviates epithelial barrier dysfunction via inhibiting apoptosis and caspase-1-mediated pyroptosis in experimental colitis. Front Pharmacol 13:849014 (2022). PubMed: 36120344
- Hao T et al. LINC-PINT suppresses tumour cell proliferation, migration and invasion through targeting miR-374a-5p in ovarian cancer. Cell Biochem Funct 38:1089-1099 (2020). PubMed: 32638404
