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Recombinant Human GAPDH protein (ab77109)

Reviews (1)Q&A (2)References (5)
SDS-PAGE - Recombinant Human GAPDH protein (ab77109)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Suitable for: SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human GAPDH protein
      GAPDH タンパク質・ペプチド 製品一覧

    • 精製度

      > 95 % SDS-PAGE.
      ab77109 is purified by using conventional chromatography techniques.

    • 発現系

      Escherichia coli

    • アクセッション番号

      P04406

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE

      • 予測される分子量

        36 kDa

      • 領域

        1 to 335

    特性

    Our Abpromise guarantee covers the use of ab77109 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.0154% DTT, 0.242% Tris, 0.0292% EDTA, 20% Glycerol (glycerin, glycerine)

    関連情報

    • 別名

      • 38 kDa BFA-dependent ADP-ribosylation substrate
      • aging associated gene 9 protein
      • Aging-associated gene 9 protein
      • BARS-38
      • cb609
      • EC 1.2.1.12
      • Epididymis secretory sperm binding protein Li 162eP
      • G3P_HUMAN
      • G3PD
      • G3PDH
      • GAPD
      • GAPDH
      • Glyceraldehyde 3 phosphate dehydrogenase
      • glyceraldehyde 3-PDH
      • Glyceraldehyde-3-phosphate dehydrogenase
      • HEL-S-162eP
      • KNC-NDS6
      • MGC102544
      • MGC102546
      • MGC103190
      • MGC103191
      • MGC105239
      • MGC127711
      • MGC88685
      • OCAS, p38 component
      • OCT1 coactivator in S phase, 38-KD component
      • peptidyl cysteine S nitrosylase GAPDH
      • Peptidyl-cysteine S-nitrosylase GAPDH
      • wu:fb33a10
      see all

    • 機能

      Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

    • パスウェイ

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

    • 配列類似性

      Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

    • 翻訳後修飾

      S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
      ISGylated.

    • 細胞内局在

      Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human GAPDH protein (ab77109)
      SDS-PAGE - Recombinant Human GAPDH protein (ab77109)
      SDS-PAGE showing 3ug of ab77109 migrating at approximately 36kDa.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (5)

    ab77109 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab77109 は 5 報の論文で使用されています。

    • Luo M  et al. Evaluating a Panel of Autoantibodies Against Tumor-Associated Antigens in Human Osteosarcoma. Front Genet 13:872253 (2022). PubMed: 35547257
    • Chen JJ  et al. DHHC5 Mediates ß-Adrenergic Signaling in Cardiomyocytes by Targeting Ga Proteins. Biophys J 118:826-835 (2020). PubMed: 31547976
    • Backlund M  et al. Endoplasmic reticulum stress increases AT1R mRNA expression via TIA-1-dependent mechanism. Nucleic Acids Res 44:3095-104 (2016). Human . PubMed: 26681690
    • Laothong U  et al. Melatonin induces apoptosis in cholangiocarcinoma cell lines by activating the reactive oxygen species-mediated mitochondrial pathway. Oncol Rep 33:1443-9 (2015). PubMed: 25606968
    • Putra SE  et al. Dealing with large sample sizes: comparison of a new one spot dot blot method to western blot. Clin Lab 60:1871-7 (2014). PubMed: 25648029

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    GAPDH with Human Samples

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    It is very good loading control for Human samples. I tried it on Human Brain tissue samples and it showed very nice specific bands at around 38KDa
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 05 2015

    Question

    I would like to enquire about the product Glutaredoxin 1 protein (Active) (ab63231). Is it recombinant human glutaredoxin 1? What is the activity of the enzyme (units/ml)?

    Read More
    Answer

    The Glutaredoxin 1 protein (Active), ab63231, is the Glutaredoxin 1 protein from Saccharomyces cerevisiae (SwissProt reference P25373).
    The activity of the protein was ascertained by monitoring its ability to oxidize NADPH. The activity was calculated to be 3X10^-3 micromol/min. The reaction mix contained NADPH (0.4 mM), GSH (1.0 mM), glutathione reductase (17 ug) and HED (1.4 mM) in 0.1 M Tris HCl, pH 7.4.
    A mixed disulphide between HED and GSH is formed within 2 minutes, and the reaction was started by the addition of 17 ug Grx1. The reaction was followed by the decrease in A340 due to the oxidation of NADPH.

    Read More

    Question

    I would like to enquire the difference between these two products:

    GAPDH protein (active) (ab82633)

    GAPDH protein (human) (ab77109)

    I am looking for an active GAPDH protein. Please advise which is suitable.

    Read More
    Answer

    Thank you for contacting us and your interest in our products.

    The GAPDH protein ab82633 and ab77109 are both the full length GAPDH expressed in E. coli, however only ab82633 has been tested for its enzymatic activity. The specific activity was found to be 35.7 U/mg. One unit of activity of GAPDH is defined as the amount of enzyme required to convert 1 µmole of NAD to NADH per minute under standard assay conditions.

    Read More

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