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Recombinant Human gamma Tubulin protein (denatured) (ab115710)

Submit a review Q&A (2)References (1)
SDS-PAGE - Recombinant Human gamma Tubulin protein (denatured) (ab115710)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human gamma Tubulin protein (denatured)

    • 精製度

      > 85 % SDS-PAGE.

    • 発現系

      Escherichia coli

    • アクセッション番号

      P23258

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MGSSHHHHHHSSGLVPRGSHMPREIITLQLGQCGNQIGFEFWKQLCAEHG ISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNS PYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSD SLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSD VVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQ LVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQ SVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDP TQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMAN HTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQ LIDEYHAATRPDYISWGTQEQ

      • 予測される分子量

        53 kDa including tags

      • 領域

        1 to 451

      • タグ

        His tag N-Terminus

    • 製品の詳細

      Recombinant Human gamma Tubulin protein

    特性

    Our Abpromise guarantee covers the use of ab115710 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 6% Urea, 0.32% Tris HCl, 5% Glycerol (glycerin, glycerine)

    関連情報

    • 別名

      • Gamma 1 tubulin
      • Gamma 2 tubulin
      • Gamma Tubulin 1
      • Gamma Tubulin 2
      • Gamma tubulin complex component 1
      • Gamma-2-tubulin
      • GCP 1
      • GCP-1
      • GCP1
      • MGC131994
      • TBG2_HUMAN
      • TUBG
      • TUBG1
      • TUBG2
      • TUBGCP1
      • Tubulin gamma 1 chain
      • Tubulin gamma 2 chain
      • Tubulin gamma complex-associated protein 1
      • Tubulin gamma-2 chain
      • tubulin, gamma 1
      • tubulin, gamma 2
      • tubulin, gamma polypeptide
      • Xgam
      see all

    • 機能

      Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.

    • 配列類似性

      Belongs to the tubulin family.

    • 翻訳後修飾

      Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.

    • 細胞内局在

      Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human gamma Tubulin protein (denatured) (ab115710)
      SDS-PAGE - Recombinant Human gamma Tubulin protein (denatured) (ab115710)
      15% SDS-PAGE showing ab115710 at approximately 53.3kDa (3µg).

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab115710 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab115710 は 1 報の論文で使用されています。

    • Larsson VJ  et al. Mitotic spindle assembly and ?-tubulin localisation depend on the integral nuclear membrane protein Samp1. J Cell Sci 131:N/A (2018). PubMed: 29514856

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    I Have recently purchase the above product. I have noticed on a gel there are 3 contaminates above 100KDa. Do you know what these are? They seem to be reacting with a anti gamma tubulin antibody (from another supplier). I am thinking they are aggregates but wondered if you have investigated this.

    Thanks

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.

    The Lab has retested this protein and it is very likely that the higher band at 100 kDa represents the multimer forms. I have attached the image to this response and hope that you can open this file. As you can see, without DTT in the SDS sample buffer, the protein didn’t move properly through the gel matrix. With the DTT, longer boiling time yielded better results i.e. less of the multimer bands and a stronger 53 kDa band.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Question

    I Have recently purchase the above product. I have noticed on a gel there are 3 contaminates above 100KDa. Do you know what these are? They seem to be reacting with a anti gamma tubulin antibody (from another supplier). I am thinking they are aggregates but wondered if you have investigated this.



    Thanks

    Read More
    Answer

    I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

    Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could provide the batch number and attach an image to the response.

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

    Read More

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