AB223021

Recombinant Human Folate Binding Protein/FBP (His tag C-Terminus)

Name: Recombinant Human Folate Binding Protein/FBP (His tag) (ab223021) | Abcam
Brand: Abcam Limited
SKU: AB223021
Price: 108000 JPY
Availability: InStock

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Recombinant Human Folate Binding Protein/FBP (His tag C-Terminus) is a Human Fragment protein, in the 26 to 234 aa range, expressed in Baculovirus infected insect cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

別名を表示する

FOLR, FOLR1, Folate receptor alpha, FR-alpha, Adult folate-binding protein, Folate receptor 1, KB cells FBP, Ovarian tumor-associated antigen MOv18, FBP

1 Images

SDS-PAGE - Recombinant Human Folate Binding Protein/FBP (His tag C-Terminus) (AB223021)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Folate Binding Protein/FBP (His tag C-Terminus) (AB223021)

3 μg of ab223021 analyzed by 15% SDS-PAGE.

28-40kDa (under reducing conditions).

Key facts

精製度

>90% SDS-PAGE

エンドトキシンレベル

< 1 EU/µg

発現系

Baculovirus infected insect cells

アプリケーション

SDS-PAGE

applications

生物活性

アクセッション番号

P15328

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Previously labelled as Folate Binding Protein

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配列情報

[{"sequence":"ADLIAWARTELLNVCMNAKHHKEKPGPEDKLHEQCRPWRKNACCSTNTSQEAHKDVSYLYRFNWNHCGEMAPACKRHFIQDTCLYECSPNLGPWIQQVDQSWRKERVLNVPLCKEDCEQWWEDCRTSYTCKSNWHKGWNWTSGFNKCAVGAACQPFHFYFPTPTVLCNEIWTHSYKVSNYSRGSGRCIQMWFDPAQGNPNEEVARFYAAAMSHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"25.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":234,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"P15328","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Folate Binding Protein (FBP) also known as FolR1 protein or FOLR-1 is a significant protein with a mass of about 32-38 kDa. It binds folate with high affinity and facilitates its transport into the cell. FBP can be found in various tissues with notable expression in the placenta kidney and certain epithelial tissues. The protein mediates folate uptake by capturing folates in the extracellular matrix and channeling them through receptor-mediated endocytosis.

Biological function summary

FBP plays a critical role in cellular growth and replication by ensuring sufficient folate supply. It belongs to a family of receptors not forming part of a larger protein complex. FBP's primary function is delivering folate an important B vitamin which acts as a one-carbon donor in DNA synthesis and repair. Optimal folate transport by FBP supports cellular functions particularly in rapidly dividing cells.

Pathways

FBP significantly contributes to the folate-mediated one-carbon metabolic pathway critical for nucleotide biosynthesis. This pathway interconnects with the homocysteine remethylation cycle. FBP associates with other enzymes such as dihydrofolate reductase (DHFR) and methylenetetrahydrofolate reductase (MTHFR) which also play key roles in these pathways. These interactions ensure efficient folate metabolism and maintenance of normal cellular functions.

FBP's relation to cancer and neural tube defects is well documented. Overexpression of FBP has been observed in various cancers including ovarian and breast cancer which indicates its potential role in tumor progression. In neural tube defects altered folate transport due to variations in FBP expression impacts neural development. FBP connects to other markers like HER2 in cancer which may form part of a therapeutic target strategy.

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製品の性状

製品の状態

Liquid

補足情報

Affinity purified

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一般的な情報

機能

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed : 19074442, PubMed : 23851396, PubMed : 23934049, PubMed : 2527252, PubMed : 8033114, PubMed : 8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed : 23851396, PubMed : 23934049, PubMed : 2527252, PubMed : 8033114, PubMed : 8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed : 8567728). Required for normal embryonic development and normal cell proliferation (By similarity).

配列の類似性

Belongs to the folate receptor family.

翻訳後修飾

The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.

細胞内局在性

Endosome

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information FOLR1

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Abcam product promise

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Recombinant Human Folate Binding Protein/FBP (His tag C-Terminus)