AB283483

Recombinant human FGF21 protein (Active)

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Recombinant human FGF21 protein (Active) is a Human Full Length protein, in the 29 to 209 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, Cell Culture, HPLC.

別名を表示する

UNQ3115/PRO10196, FGF21, Fibroblast growth factor 21, FGF-21

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Mass Spectrometry - Recombinant human FGF21 protein (Active) (AB283483)

Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human FGF21 protein (Active) (AB283483)

Mass determination by ESI-TOF.

Predicted MW is 19467.90 Da (+/- 10 Da by ESI-TOF). Observed MW is 19469.16

Functional Studies - Recombinant human FGF21 protein (Active) (AB283483)

FuncS

Supplier Data

Functional Studies - Recombinant human FGF21 protein (Active) (AB283483)

Functional analysis of ab283483.

Fully biologicaly activr determined by dose dependent proliferation of of NIH/3T3 cells in the presence of mouse β-Klotho and heparin.

ED₅₀ for this effect is ≤484.6 ng/ml, corresponding to a specific activity of 2.06 x 10³ units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot GR3395757-1

HPLC - Recombinant human FGF21 protein (Active) (AB283483)

HPLC

Supplier Data

HPLC - Recombinant human FGF21 protein (Active) (AB283483)

HPLC analysis of ab283483

SDS-PAGE - Recombinant human FGF21 protein (Active) (AB283483)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human FGF21 protein (Active) (AB283483)

SDS-page analysis of ab283483

Key facts

精製度

>95% SDS-PAGE

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

アプリケーション

Mass Spec, FuncS, HPLC, Cell Culture, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Fully biologicaly activr determined by dose dependent proliferation of of NIH/3T3 cells in the presence of mouse β-Klotho and heparin.

ED₅₀ for this effect is ≤484.6 ng/ml, corresponding to a specific activity of 2.06 x 10³ units/mg.

アクセッション番号

Q9NSA1

アニマルフリー

Yes

キャリアフリー

種

Human

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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配列情報

[{"sequence":"HPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS","proteinLength":"Full Length","predictedMolecularWeight":"19.47 kDa","actualMolecularWeight":"19.47 kDa","aminoAcidEnd":209,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9NSA1","tags":[]}]

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

Ambient

長期保存温度

Ambient

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.

Biological function summary

FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.

Pathways

FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.

FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.

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製品の性状

製品の状態

Lyophilized

補足情報

>95% by HPLC

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一般的な情報

機能

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.

配列の類似性

Belongs to the heparin-binding growth factors family.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information FGF21

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Recombinant human FGF21 protein (Active)