Recombinant human FGF20 protein (ab50022)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human FGF20 protein -
生理活性
Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 5 ng/ml in the presence of 1 µg/ml heparin, corresponding to a specific activity of ≥ 2 x 105 units/mg.
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精製度
> 90 % SDS-PAGE.
Greater than 90% by SDS-PAGE and HPLC analyses. -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
PLAEVGGFLG GLEGLGQQVG SHFLLPPAGE RPPLLGERRS AAERSARGGP GAAQLAHLHG ILRRRQLYCR TGFHLQILPD GSVQGTRQDH SLFGILEFIS VAVGLVSIRG VDSGLYLGMN DKGELYGSEK LTSECIFREQ FEENWYNTYS SNIYKHGDTG RRYFVALNKD GTPRDGARSK RHQKFTHFLP RPVDPERVPE LYKDLLMYT -
予測される分子量
23 kDa -
領域
3 to 211
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特性
Our Abpromise guarantee covers the use of ab50022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.3% Tris, 8.7% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Centrifuge the vial prior to opening. Reconstitute in 10mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex. Store at 2°C to 8°C for 1 week, or prepare for extended storage. For extended storage follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA). Store working aliquots at -20°C to -80°C.
関連情報
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別名
- FGF-20
- Fgf20
- FGF20_HUMAN
see all -
機能
Neurotrophic factor that regulates central nervous development and function. -
組織特異性
Predominantly expressed in the cerebellum. -
配列類似性
Belongs to the heparin-binding growth factors family. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab50022 は論文での使用が確認できていません。