AB285462

Recombinant human FGF2 protein (Active)

Bioactive
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Recombinant human FGF2 protein (Active) is a Human Full Length protein, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for FuncS.

別名を表示する

FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2

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Functional Studies - Recombinant human FGF2 protein (Active) (AB285462)

FuncS

Supplier Data

Functional Studies - Recombinant human FGF2 protein (Active) (AB285462)

The ED₅₀ as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 30 ng/ml.

Key facts

精製度

>95% SDS-PAGE

エンドトキシンレベル

< 1 EU/µg

発現系

Escherichia coli

アプリケーション

FuncS

applications

生物活性

Yes

生物学的活性

The ED50, calculated by the dose-dependent proliferation of BALB/c3T3 cells is typically <100ng/ml.

アクセッション番号

P09038

アニマルフリー

Yes

キャリアフリー

Yes

種

Human

再構成

Reconstitute in 5 mM Tris, pH 7.6

バッファー組成

pH: 7.6 Constituents: 0.87% Sodium chloride, 0.06% Tris

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

This product is manufactured by BioVision, an Abcam company and was previously called 4037 FGF-2/FGF-basic, human recombinant. 4037-50 is the same size as the 50 μg size of ab285462.

Lyophilized FGF2 is best-stored desiccated below 0°C.

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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"17.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P09038","tags":[]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FGF2 also known as FGF-2 or basic fibroblast growth factor is a protein that plays important roles in various cellular processes. It is a member of the fibroblast growth factor family and has a molecular weight of approximately 18 kDa. Scientists find FGF2 protein in many tissues including brain myocardium and bone. The protein can exist in different forms including recombinant variants. It interacts with cell surface receptors to exert its effects significantly influencing cell behavior.

Biological function summary

Various functions characterize FGF2 such as cell proliferation differentiation and migration. It does not act alone but is part of a larger protein complex. This complex includes FGF receptors which help mediate the binding of the FGF2 peptide ensuring proper signaling within the cells. FGF2 is important for development and repair processes making it an essential factor in tissue maintenance and regeneration.

Pathways

FGF2 plays a big role in critical pathways like the MAPK/ERK signaling pathway and PI3K/Akt pathway. These pathways help regulate the processes of cell growth and survival. FGF2 interacts with other protein molecules such as FGFR1 and FGFR2 which serve as receptors to propagate the signaling cascade initiated by FGF2 binding. Its interaction in these pathways underlines its role in modulating important cellular responses.

Scientists connect FGF2 with conditions such as cancer and atherosclerosis. The protein's deregulation may lead to abnormal cell proliferation contributing to tumor growth and vascular diseases. FGF2's interaction with proteins like FGFR1 can influence cancer progression and plaque formation in blood vessels. By understanding FGF2's role in these diseases researchers aim to develop targeted therapies that can potentially alter disease outcomes.

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed : 28302677). Binds to integrin ITGAV : ITGB3 (PubMed : 28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed : 28302677, PubMed : 8663044). Functions as a potent mitogen in vitro (PubMed : 1721615, PubMed : 3732516, PubMed : 3964259). Can induce angiogenesis (PubMed : 23469107, PubMed : 28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed : 29501879).

配列の類似性

Belongs to the heparin-binding growth factors family.

翻訳後修飾

Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information FGF2

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant human FGF2 protein (Active)