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AB132942

Recombinant Human Ezrin protein (GST tag N-Terminus)

Recombinant Human Ezrin protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human Ezrin protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 586 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

別名を表示する

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

1 Images
SDS-PAGE - Recombinant Human Ezrin protein (GST tag N-Terminus) (AB132942)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Ezrin protein (GST tag N-Terminus) (AB132942)

12.5% SDS-PAGE analysis of ab132942 stained with Coomassie Blue

Key facts

発現系

Wheat germ

タグ

GST tag N-Terminus

アプリケーション

ELISA, WB, SDS-PAGE

applications

生物活性

No

アクセッション番号

P15311

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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組合せ製品

Primary Antibodies

AB40839

Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (AB40839)

  • 5
  • 5
Proteins & Peptides

AB153408

Recombinant Human Septin 11 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Septin 11 protein (GST tag N-Terminus) (AB153408)

  • 0
  • 0
Primary Antibodies

AB52490

Anti-Moesin antibody [EP1863Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Moesin antibody [EP1863Y] (AB52490)

  • 5
  • 5
Primary Antibodies

AB76020

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (AB76020)

  • 5
  • 18
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL","proteinLength":"Full Length","predictedMolecularWeight":"89.98 kDa","actualMolecularWeight":null,"aminoAcidEnd":586,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P15311","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Proprietary technique
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.
Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.
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一般的な情報

機能

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

翻訳後修飾

Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

細胞内局在性

Cytoskeleton

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製品プロトコール

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ターゲットの情報

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
See full target information EZR
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular oncology 17:1628-1647 PubMed37171030

2023

Pancreatic cancer-derived small extracellular vesical ezrin activates fibroblasts to exacerbate cancer metastasis through STAT3 and YAP-1 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ting Chang,Hsuan-Yu Peng,Chun-Mei Hu,Sui-Chih Tien,Yi-Ing Chen,Yung-Ming Jeng,Ming-Chu Chang
View all publications
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