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Recombinant Human Estrogen Receptor alpha protein (ab82606)

Submit a review Q&A (3)References (4)
SDS-PAGE - Recombinant Human Estrogen Receptor alpha protein (ab82606)
  • Western blot - Recombinant Human Estrogen Receptor alpha protein (ab82606)

Key features and details

  • Expression system: Baculovirus infected insect cells
  • Purity: > 90% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, WB

製品の詳細

  • 製品名

    Recombinant Human Estrogen Receptor alpha protein
    Estrogen Receptor alpha タンパク質・ペプチド 製品一覧

  • 精製度

    > 90 % SDS-PAGE.
    ab82606 is greater than 90% homogeneous and based on SDS-PAGE analysis, purified by affinity and FPLC chromatography.

  • 発現系

    Baculovirus infected insect cells

  • アクセッション番号

    NM_000125

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      MTMTLHTKAS GMALLHQIQG NELEPLNRPQ LKIPLERPLG EVYLDSSKPA VYNYPEGAAY EFNAAAAANA QVYGQTGLPY GPGSEAAAFG SNGLGGFPPL NSVSPSPLML LHPPPQLSPF LQPHGQQVPY YLENEPSGYT VREAGPPAFY RPNSDNRRQG GRERLASTND KGSMAMESAK ETRYCAVCND YASGYHYGVW SCEGCKAFFK RSIQGHNDYM CPATNQCTID KNRRKSCQAC RLRKCYEVGM MKGGIRKDRR GGRMLKHKRQ RDDGEGRGEV GSAGDMRAAN LWPSPLMIKR SKKNSLALSL TADQMVSALL DAEPPILYSE YDPTRPFSEA SMMGLLTNLA DRELVHMINW AKRVPGFVDL TLHDQVHLLE CAWLEILMIG LVWRSMEHPG KLLFAPNLLL DRNQGKCVEG MVEIFDMLLA TSSRFRMMNL QGEEFVCLKS IILLNSGVYT FLSSTLKSLE EKDHIHRVLD KITDTLIHLM AKAGLTLQQQ HQRLAQLLLI LSHIRHMSNK GMEHLYSMKC KNVVPLYDLL LEMLDAHRLH APTSRGGASV EETDQSHLAT AGSTSSHSLQ KYYITGEAEG FPATV

    • 予測される分子量

      68 kDa

    • 領域

      1 to 595

    • タグ

      His tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab82606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Western blot

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.9
    Constituents: 20% Glycerol (glycerin, glycerine), 0.00584% EDTA, 0.316% Tris HCl, 0.0154% DTT, 0.75% Potassium chloride

関連情報

  • 別名

    • 7*/654 isoform
    • 7*/819 2 isoform
    • 7*/822 isoform
    • 8*/901 isoform
    • 8*/941 isoform
    • DKFZp686N23123
    • ER
    • ER alpha
    • ER-alpha
    • Era
    • ESR
    • ESR1
    • ESR1_HUMAN
    • ESRA
    • Estradiol receptor
    • Estrogen nuclear receptor alpha
    • Estrogen receptor
    • Estrogen receptor 1
    • Estrogen receptor alpha
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform
    • Estrogen receptor alpha delta 4 +49 isoform
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform
    • Estrogen receptor alpha E1 E2 1 2
    • Estrogen receptor alpha E1 N2 E2 1 2
    • ESTRR
    • NR3A1
    • Nuclear receptor subfamily 3 group A member 1
    see all

  • 機能

    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • ドメイン

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • 翻訳後修飾

    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).

  • 細胞内局在

    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt

画像

  • SDS-PAGE - Recombinant Human Estrogen Receptor alpha protein (ab82606)
    SDS-PAGE - Recombinant Human Estrogen Receptor alpha protein (ab82606)
  • Western blot - Recombinant Human Estrogen Receptor alpha protein (ab82606)
    Western blot - Recombinant Human Estrogen Receptor alpha protein (ab82606)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (4)

ab82606 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab82606 は 4 報の論文で使用されています。

  • Arcadio F  et al. Estradiol Detection for Aquaculture Exploiting Plasmonic Spoon-Shaped Biosensors. Biosensors (Basel) 13:N/A (2023). PubMed: 37185507
  • Vanzan L  et al. High throughput screening identifies SOX2 as a super pioneer factor that inhibits DNA methylation maintenance at its binding sites. Nat Commun 12:3337 (2021). PubMed: 34099689
  • Tian Z  et al. TRIM8 inhibits breast cancer proliferation by regulating estrogen signaling. Am J Cancer Res 10:3440-3457 (2020). PubMed: 33163282
  • Xue M  et al. Regulation of estrogen signaling and breast cancer proliferation by an ubiquitin ligase TRIM56. Oncogenesis 8:30 (2019). PubMed: 31000690

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

Dear technical team,

Our customer would like to purchase ab82606 and it is His tagged recombinant protein.
https://www.abcam.com/Estrogen-Receptor-alpha-protein-His-tag-ab82606.html
She would like to know where His were tagged on, N-terminal or C-terminal.
Please let me know.

Best regards

Read More
Answer

Thank you for your reply.

I am happy to confirm that ab82606 has a 6x HIS tag at the N-terminus.

I hope this information is helpful for your customer and wish them good luck with their research.

Read More

Question

Dear Sir/Madam

I am interested in the estrogen receptor alpha and progesterone receptor products you have in your catalogue. Specifally items ab82606 (ER) and ab75958 (PR). I want to use these in a new assay I am developing to look at ligand binding interactions. Therefore I am interested to know if these products will be suitable for looking at ligand binding. Do they possess the appropriate domain? I am particulary concerned in the PR case because there is no full length protein.

Alternatively can you suggest an alternative product that would be suitable for the assay I want to perform?

Man thanks

Read More
Answer

Thank you for contacting us and sorry for the delay in getting back to you.

We have no record of the estrogen receptor alpha (ab82606) and progesterone receptor (ab75958) proteins being used in the assays you have described. The estrogen receptor alpha (ab82606) protein is the full length recombinant protein expressed in a baculovirus system. Although we have not tested it in any functional assays as yet, and we have no reports of our customers having done so either, it may be that the protein is compatible with ligand binding assays but I would not be able to guarantee this.

If you would like to try this protein in your ligand binding assays I would be able to offer you a testing discount. This offer means that if purchase ab82606 andtest theprotein for itsligand binding and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for the equivalent price of ab82606 off any product in our catalogue. More information on this offer can be found here:

https://www.abcam.com/collaboratordiscount

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab82606.

I think the Progesterone Receptor (ab75958) protein is less likely to work in your assays. This is because the product is a truncation of the Progesterone Receptor (residues 412-562, SwissProt reference P06401). This region does not include the purportedsteroid binding region (residues 681-933). I would therefore not expect the protein to be useful in your assays.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Question

Hi Estrogen Receptor alpha protein (His tag) Abcam ab82606 ..( 4µg) We have ordered the above product and would like to know the following things Protein-protein interaction assay: Use 100 units (how to convert the protein in mg to 100 units) Is there any abcam protocol available for Protein-protein interaction assay

Read More
Answer

Thank you for contacting Abcam. For your first question, 1 unit is equal to 1 nanogram of purified protein. As to Abcam protocols for protein-protein interaction, we do not have our own protocols but I did find the following link that may be useful: http://www.promega.com/˜/media/files/resources/product%20guides/protein%20interaction/chap3.ashx?la=en Please let me know if there is anything else I can help you with.

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