Recombinant Human ERK2 protein (ab43624)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Densitometry
- Suitable for: WB
製品の詳細
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製品名
Recombinant Human ERK2 protein
ERK2 タンパク質・ペプチド 製品一覧 -
精製度
> 90 % Densitometry. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Recombinant Protein
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Related Products
特性
Our Abpromise guarantee covers the use of ab43624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
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製品の状態
Liquid -
備考
ab43624 (Human ERK2 full length protein) can be utilized as a substrate for the active protein kinase ab60013 (Active human MEK2 full length protein)
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
関連情報
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別名
- ERK 2
- ERK-2
- ERT1
see all -
機能
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
ドメイン
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻訳後修飾
Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
細胞内局在
Nucleus. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human ERK2 protein (ab43624)The purity of ab43624 was determined to be >90% by SDS-PAGE. MW ~68kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab43624 は論文での使用が確認できていません。