Recombinant human ERK1 protein (ab105904)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies
製品の詳細
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製品名
Recombinant human ERK1 protein
ERK1 タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity 840 nmol/min/mg.
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精製度
> 90 % Densitometry.
ab105904 was determined to be >90% pure densitometry. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
72 kDa including tags -
領域
1 to 379 -
タグ
GST tag N-Terminus
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab105904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
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製品の状態
Liquid -
備考
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- ERK 1
- ERK-1
- ERK1
see all -
機能
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
ドメイン
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻訳後修飾
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
画像
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Functional Studies - Recombinant human ERK1 protein (ab105904)The specific activity of ERK1 (ab105904) was determined to be 715 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human ERK1 protein (ab105904)SDS PAGE analysis of ab105904 -
SDS-PAGE - Recombinant human ERK1 protein (ab105904)SDS PAGE analysis of ab105904 -
SDS-PAGE - Recombinant human ERK1 protein (ab105904)ab105904 was determined to be >90% pure by densitometry. -
Functional Studies - Recombinant human ERK1 protein (ab105904)The specific activity of ab105904 was determined to be 840 nmol/min/mg.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab105904 は論文での使用が確認できていません。