Recombinant human EPO protein (Active) (ab283908)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE, MS
製品の詳細
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製品名
Recombinant human EPO protein (Active)
EPO タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent proliferation of TF-1 cells.
ED50 is ≤ 0.29ng/ml, corresponding to a specific activity of 3.43 x 106units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters and meet the same parameters as the first lot.
GR3432989-2.
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精製度
>= 95 % HPLC.
SDS-PAGE = 95% Purity -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
APPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYA WKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVS GLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLR GKLKLYTGEACRTGDR -
予測される分子量
18 kDa -
実際の分子量
18 kDa -
分子量情報
Predicted MW is 18453 Da (+/- 10 Da by ESI-TOF). Observed MW is 18666.27 Da. -
領域
28 to 193 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide and pro-peptide.
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特性
Our Abpromise guarantee covers the use of ab283908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
HPLC
SDS-PAGE
Mass Spectrometry
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phophate buffered saline, aliquot and store at -80 °C for 12 months or +4 °C for 1 week. Avoid repeated freeze-thaw.
関連情報
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別名
- EP
- EPO
- EPO alpha
see all -
関連性
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
細胞内局在
Secreted
画像
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Functional Studies - Recombinant human EPO protein (Active) (ab283908)
Fully biologically active as determined by the dose dependent proliferation of TF-1 cells. ED50 for this effect is ≤0.29 ng/ml corresponding to a specific activity of 3.43 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters and meet the same parameters as the first lot.
GR3432989-2.
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Mass Spectrometry - Recombinant human EPO protein (Active) (ab283908)Mass determination by ESI-TOF.
Predicted MW is 18453 Da (+/- 10 Da by ESI-TOF). Observed MW is 18666.27 Da.
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HPLC - Recombinant human EPO protein (Active) (ab283908)HPLC analysis of ab283908.
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SDS-PAGE - Recombinant human EPO protein (Active) (ab283908)SDS-PAGE analysis of ab283908.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab283908 は論文での使用が確認できていません。
