Recombinant human Enterokinase protein (Active) (ab245805)
Key features and details
- Expression system: CHO cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
製品の詳細
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製品名
Recombinant human Enterokinase protein (Active)
Enterokinase タンパク質・ペプチド 製品一覧 -
生理活性
Sequentially cleaves carboxyl side of D-D-D-D-K.
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精製度
> 90 % SDS-PAGE.
>90 % by HPLC. -
発現系
CHO cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
109 kDa -
配列の追加情報
Consists of two polypeptide chains (784 aa heavy chain and 235 aa light chain) held together by a single disulfide bond, resulting in formation of a biologically active heterodimer.
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特性
Our Abpromise guarantee covers the use of ab245805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
HPLC
SDS-PAGE
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製品の状態
Lyophilized -
備考
Human Enterokinase is expressed as a linear 1019 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of Enterokinase, which consists of two polypeptide chains (heavy chain and light chain) held together by a single disulfide bond, resulting in formation of a biologically active heterodimer. The heavy chain consists of 784 amino acid residues, and the light chain consists of 235 amino acid residues.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.16% Sodium phosphate, 0.01% Calcium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute in water to 0.1-1.0 mg/ml.
関連情報
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別名
- PRSS 7
- PRSS7
- Enterokinase
see all -
機能
Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. -
組織特異性
Intestinal brush border. -
関連疾患
Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD) [MIM:226200]; a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. -
配列類似性
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 2 LDL-receptor class A domains.
Contains 1 MAM domain.
Contains 1 peptidase S1 domain.
Contains 1 SEA domain.
Contains 1 SRCR domain. -
翻訳後修飾
The chains are derived from a single precursor that is cleaved by a trypsin-like protease. -
細胞内局在
Membrane. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245805 は論文での使用が確認できていません。