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Recombinant Human eNOS protein (ab112329)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human eNOS protein (ab112329)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: ELISA, SDS-PAGE, WB

    製品の詳細

    • 製品名

      Recombinant Human eNOS protein
      eNOS タンパク質・ペプチド 製品一覧

    • 発現系

      Wheat germ

    • アクセッション番号

      P29474

    • タンパク質長

      Protein fragment

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        QPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQ GRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAAT

      • 予測される分子量

        37 kDa including tags

      • 領域

        61 to 160

      • タグ

        GST tag N-Terminus

    特性

    Our Abpromise guarantee covers the use of ab112329 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      ELISA

      SDS-PAGE

      Western blot

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • cNOS
      • Constitutive NOS
      • EC NOS
      • EC-NOS
      • ecNOS
      • Endothelial nitric oxidase synthase
      • Endothelial nitric oxide synthase
      • Endothelial nitric oxide synthase 3
      • Endothelial NOS
      • eNOS
      • Nitric oxide synthase 3
      • Nitric oxide synthase 3 (endothelial cell)
      • Nitric oxide synthase 3 endothelial cell
      • Nitric oxide synthase endothelial
      • Nitric oxide synthase, endothelial
      • NOS 3
      • NOS III
      • NOS type III
      • NOS3
      • NOS3_HUMAN
      • NOSIII
      see all

    • 機能

      Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
      Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

    • 組織特異性

      Platelets, placenta, liver and kidney.

    • 関連疾患

      Variation in NOS3 seem to be associated with susceptibility to coronary spasm.

    • 配列類似性

      Belongs to the NOS family.
      Contains 1 FAD-binding FR-type domain.
      Contains 1 flavodoxin-like domain.

    • 翻訳後修飾

      Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.

    • 細胞内局在

      Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human eNOS protein (ab112329)
      SDS-PAGE - Recombinant Human eNOS protein (ab112329)
      ab112329 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab112329 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab112329 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    We have a customer who is looking for recombinant protein to use as positive control in western blot for eNOS/NOS3, eNOS/NOS3 phosphorylated on serine 1177, DDAH1 and DDAH2. Do you have this product?

    Read More
    Answer

    Thank you for contacting us.

    We have:

    DDAH1 protein (https://www.abcam.com/ab99863), Recombinant full length Human DDAH1 (amino acids 1-285) with an N terminal His tag.
    DDAH2 protein (Tagged-His Tag) (https://www.abcam.com/ab92017), Recombinant fragment, corresponding to amino acids 71-261 (191 amino acids) of Human DDAH2 with N terminal His tag.



    For eNOS, the only 2 products we have are:

    The protein fragment https://www.abcam.com/ab112329, corresponding to amino acids 61-160 of Human eNOS with a proprietary tag.
    The peptide https://www.abcam.com/ab109090, immunogen for ab95254.



    The 2 eNOS products are not phosphorylated.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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