Recombinant human EHMT2/G9A protein (ab80323)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human EHMT2/G9A protein
EHMT2/G9A タンパク質・ペプチド 製品一覧 -
生理活性
Activity: 22 pmol/min/µg. Assay conditions: Enzyme was incubated with 0.5 µM of biotinylated H3 peptide with 40 µM AdoMet in HMT buffer 1A in a 10-µl reaction. 5 µl of Anti-Mouse AlphaLISA acceptor beads (1:250) and 5 µl of Primary Antibody 7 (1:50) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 µl) and mixture was further incubated for 15 min at RT before counts were measured.
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精製度
> 80 % SDS-PAGE.
Affinity purified. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
75 kDa including tags -
領域
785 to 1210 -
タグ
GST tag N-Terminus -
配列の追加情報
74.6 kDa including tag
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特性
Our Abpromise guarantee covers the use of ab80323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Ankyrin repeat containing protein
- Bat 8
- Bat8
see all -
機能
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. -
組織特異性
Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. -
配列類似性
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 7 ANK repeats.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
ドメイン
The SET domain mediates interaction with WIZ.
The ANK repeats bind H3K9me1 and H3K9me2. -
翻訳後修飾
Methylated at Lys-185; automethylated. -
細胞内局在
Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin. - Information by UniProt
画像
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SDS-PAGE - Recombinant human EHMT2/G9A protein (ab80323)
SDS-PAGE showing ab80323 at approximately 74.6kDa (5µg).
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Functional Studies - Recombinant human EHMT2/G9A protein (ab80323)Image showing specific activity of ab80323.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab80323 は論文での使用が確認できていません。