Recombinant human EEF2K protein (ab84570)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human EEF2K protein -
生理活性
Specific activity of EEF2K was determined to be 50nmol/min/mg as per activity assay protocol
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精製度
> 80 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
領域
1 to 725
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab84570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab204858 (Myosin-2 heavy chain peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- C86191
- Calcium/calmodulin dependent eukaryotic elongation factor 2
- Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase
see all -
機能
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin. -
配列類似性
Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain. - Information by UniProt
画像
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Functional Studies - Recombinant human EEF2K protein (ab84570)The specific activity of EEF2K (ab84570) was determined to be 42 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human EEF2K protein (ab84570)SDS PAGE analysis of ab84570 -
SDS-PAGE - Recombinant human EEF2K protein (ab84570)SDS PAGE analysis of ab84570 -
Functional Studies - Recombinant human EEF2K protein (ab84570)Specific activity of EEF2K was determined to be 50nmol/min/mg as per activity assay protocol. -
SDS-PAGE - Recombinant human EEF2K protein (ab84570)Purity of EEF2K was determined to be >80% by densitometry. approx. MW 125kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab84570 は論文での使用が確認できていません。