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AB99388

Recombinant Human dUTPase protein (His tag N-Terminus)

Recombinant Human dUTPase protein (His tag N-Terminus)

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Recombinant Human dUTPase protein (His tag N-Terminus) is a Human Full Length protein, in the 70 to 252 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

dUTPase, dUTP pyrophosphatase, DUT

1 Images
SDS-PAGE - Recombinant Human dUTPase protein (His tag N-Terminus) (AB99388)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human dUTPase protein (His tag N-Terminus) (AB99388)

15% SDS-PAGE analysis of 3μg ab99388.

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

SDS-PAGE, Mass Spec

applications

生物活性

No

アクセッション番号

P33316

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMASTVGAAGWKGELPKAGGSPAPGPETPAISPSKRARPAEVGGMQLRFARLSEHATAPTRGSARAAGYDLYSAYDYTIPPMEKAVVKTDIQIALPSGCYGRVAPRSGLAAKHFIDVGAGVIDEDYRGNVGVVLFNFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGSTGKN","proteinLength":"Full Length","predictedMolecularWeight":"21.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":252,"aminoAcidStart":70,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P33316","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The enzyme deoxyuridine triphosphatese also known as dUTPase hydrolyzes dUTP to dUMP and inorganic pyrophosphate. It prevents the incorporation of uracil into DNA which can lead to mutations. The molecular mass of human dUTPase is about 18-20 kDa. It is widely expressed in both prokaryotic and eukaryotic cells including places like the thymus and spleen where DNA synthesis is very active. Alternative names such as DUT or dUTP pyrophosphatase are often used to refer to this enzyme.
Biological function summary

DUTPase maintains nucleotide pool balance and genome integrity. By converting dUTP to dUMP it provides substrates for thymidylate synthase to produce thymidine triphosphate (dTTP) which is essential for DNA replication and repair. dUTPase is not typically part of a larger protein complex but cooperates with other enzymes in nucleotide metabolism. Its activity protects cells from apoptosis that might occur due to extensive uracil misincorporation.

Pathways

DUTPase plays a role in nucleotide metabolism and one-carbon metabolism pathways. It regulates the pyrimidine metabolism pathway ensuring sufficient dTTP levels for DNA synthesis. dUTPase interacts indirectly with proteins like thymidylate synthase and dihydrofolate reductase through these pathways. These interactions aid in maintaining a steady supply of nucleotides important for DNA replication and repair.

Impairments in dUTPase function can relate to cancer and immunodeficiency disorders. In some cancers altered dUTPase expression affects the sensitivity to chemotherapeutic agents particularly those targeting thymidylate synthesis. In certain immunodeficiencies dUTPase malfunction leads to increased uracil incorporation compromising DNA integrity. The enzyme connects with proteins such as thymidylate synthase in these diseases due to its role in managing nucleotide pools critical for cell proliferation.
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製品の性状

製品の状態

Liquid

補足情報

ab99388 is purified using conventional chromatography techniques.

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一般的な情報

機能

Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis (PubMed : 17880943, PubMed : 8631816, PubMed : 8805593). Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor (By similarity). Essential for embryonic development (By similarity).

配列の類似性

Belongs to the dUTPase family.

翻訳後修飾

Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis (PubMed : 17880943, PubMed : 8631816, PubMed : 8805593). Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor (By similarity). Essential for embryonic development (By similarity).
See full target information DUT
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