Recombinant Human DTYMK protein (ab109838)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
製品の詳細
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製品名
Recombinant Human DTYMK protein
DTYMK タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
No detergents such as urea, Triton or Tween have been used to purify the protein. Purified by using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S 200) with 20mM Tris pH 7.5, 2mM EDTA. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MGSSHHHHHH SSGLVPRGSH MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG KLLSSYLQKK SDVEDHSVHL LFSANRWEQV PLIKEKLSQG VTLVVDRYAF SGVAFTGAKE NFSLDWCKQP DVGLPKPDLV LFLQLQLADA AKRGAFGHER YENGAFQERA LRCFHQLMKD TTLNWKMVDA SKSIEAVHED IRVLSEDAIR TATEKPLGEL WK -
予測される分子量
24 kDa -
領域
1 to 212 -
タグ
His tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab109838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Mass Spectrometry
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質量分析
MALDI-TOF -
製品の状態
Liquid -
備考
Via its role in the catalytic creation of dTDP, DTYMK plays an important role in the pathway of DNA synthesis and is thought to be involved in cell cycle progression and cell growth.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.04% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
関連情報
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別名
- CDC8
- deoxythymidylate kinase
- Deoxythymidylate kinase (thymidylate kinase)
see all -
機能
Catalyzes the conversion of dTMP to dTDP. -
パスウェイ
Pyrimidine metabolism; dTTP biosynthesis. -
配列類似性
Belongs to the thymidylate kinase family. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human DTYMK protein (ab109838)
SDS-PAGE analysis of Recombinant Human DTYMK protein (ab109838), under reducing conditions. Proteins visualized by coomassie blue stain.
Lane 1: Molecular Weight Standards
Lane 2: 3 µg Recombinant Human DTYMK protein
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab109838 は 2 報の論文で使用されています。
- Su JY & Sclafani RA Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast. Nucleic Acids Res 19:823-7 (1991). PubMed: 2017365
- Cummins RR & Balinsky D Activities of some enzymes of pyrimidine and DNA synthesis in a rat transplantable hepatoma and human primary hepatomas, in cell lines derived from these tissues, and in human fetal liver. Cancer Res 40:1235-9 (1980). PubMed: 6244089