Recombinant Human DR5 Protein (Active) (ab290061)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS
製品の詳細
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製品名
Recombinant Human DR5 Protein (Active)
DR5 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by its ability to inhibit Trail-induced apoptosis in LN-18 cells.
ED50 is ≤ 12.7 ng/ml, corresponding to a specific activity of 7.87 x 104 units/mg.
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精製度
>= 95 % HPLC.
SDS-PAGE >= 95% -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
ALITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDY STHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMC RKCRTGCPRGMVKVGDCTPWSDIECVHKESGTKHSGEVPAVEETVTSSPG TPASPCS -
予測される分子量
17 kDa -
実際の分子量
17 kDa -
分子量情報
Predicted MW is 17211.11 Da (+/-10 Da by ESI-TOF). Observed MW is 17210.87 Da. -
領域
54 to 210 -
配列の追加情報
N-terminal glycine
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特性
Our Abpromise guarantee covers the use of ab290061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
Mass Spectrometry
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
関連情報
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別名
- Fas like protein
- Apoptosis inducing protein TRICK2A/2B
- Apoptosis inducing receptor TRAIL R2
see all -
機能
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
組織特異性
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
関連疾患
Squamous cell carcinoma of the head and neck -
配列類似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
細胞内局在
Membrane. - Information by UniProt
画像
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Biological Activity - Recombinant Human DR5 Protein (Active) (ab290061)
Fully biologically active determined by its ability to inhibit Trail-induced apoptosis in LN-18 cells.
ED50 is ≤ 12.7 ng/ml, corresponding to a specific activity of 7.87 x 104 units/mg.
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HPLC - Recombinant Human DR5 Protein (Active) (ab290061)HPLC analysis of ab290061
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Mass Spectrometry - Recombinant Human DR5 Protein (Active) (ab290061)Mass determination by ESI-TOF. Predicted MW is 17211.11 Da (+/-10 Da by ESI-TOF). Observed MW is 17210.87 Da.
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SDS-PAGE - Recombinant Human DR5 Protein (Active) (ab290061)SDS-PAGE analysis of ab290061
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab290061 は論文での使用が確認できていません。