Recombinant Human DNA Polymerase lambda protein (ab113399)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
製品の詳細
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製品名
Recombinant Human DNA Polymerase lambda protein -
精製度
> 90 % SDS-PAGE.
Conventional chromatography techniques (anion exchange followed by gel filtration with 20mM Tris pH 7.5, 2mM EDTA). -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MGSSHHHHHHSSGLVPRGSHMLMHHQKYLQRFLGGKREKKQKEACSIPGI GKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQMWYQQ GFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQKAAQA FNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSLRQEG FLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLY FTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPT EKDVFRLLGLPYREPAERDW -
予測される分子量
36 kDa -
領域
1 to 300 -
タグ
His tag N-Terminus -
配列の追加情報
The protein sequence refers to isoform 2 and contains MGSSHHHHHHSSGLVPRGSH sequence at N-Terminus for the His tag.
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製品の詳細
Recombinant Human DNA Polymerase lambda/Polk protein
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab113399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Mass Spectrometry
SDS-PAGE
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質量分析
MALDI-TOF -
製品の状態
Liquid -
備考
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
関連情報
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別名
- BETA N
- BETAN
- DNA directed DNA polymerase lambda
see all -
機能
Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
組織特異性
Expressed in a number of tissues. Abundant in testis. -
配列類似性
Belongs to the DNA polymerase type-X family.
Contains 1 BRCT domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human DNA Polymerase lambda protein (ab113399)
15% SDS-PAGE of DNA Polymerase lambda protein: (1) MW ladder, (2) protein loaded 3ug
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113399 は論文での使用が確認できていません。