Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE, WB, Functional Studies
製品の詳細
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製品名
Recombinant human DNA Polymerase Kappa/POLK protein -
精製度
> 90 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFY GNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLS NTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRA AMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEA YLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISP LLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFR IEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLP IRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISL GLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKER LKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAIAKELLKTEIDADF PHPLRLRLMGVRISSFPNEEDRKHQQRSIIGFLQAGNQALSATECTLEKT DKDKFVKPLE -
予測される分子量
64 kDa -
領域
1 to 560 -
タグ
His tag C-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab176956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
SDS-PAGE
Western blot
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C or -80°C.
pH: 6
Constituents: 1.17% Sodium chloride, 0.14% Sodium phosphate, 50% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- DinB homolog 1 (E. coli)
- DINB protein
- DINB1
see all -
機能
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. -
組織特異性
Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes. -
配列類似性
Belongs to the DNA polymerase type-Y family.
Contains 2 UBZ-type zinc fingers.
Contains 1 umuC domain. -
ドメイン
The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. -
細胞内局在
Nucleus. Detected throughout the nucleus and at replication foci. - Information by UniProt
画像
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Functional Studies - Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)
DNA polymerizing activity of ab176956
50 mM Tris-HCl pH 8.0, 2 mM MgCl2, 1 mM DTT, 0.1 mg / ml BSA, 0.1 mM dNTP, 100 nM primer/template(13-mer/30-mer), DNA polymerase kappa/POLK at 13~50 ng/25μl (lanes 1,2, and 3 respectiviely) per assay, incubated at 37℃ for 10 min. -
SDS-PAGE - Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)SDS-PAGE analysis of ab196956.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab176956 は論文での使用が確認できていません。