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Recombinant human DLL4 protein (ab84081)

Submit a review Q&A (1)References (1)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

製品の詳細

  • 製品名

    Recombinant human DLL4 protein
    DLL4 タンパク質・ペプチド 製品一覧

  • 生理活性

    Biological Activity : ab84081, when immobilized at concentrations >1.5 µg/mL will inhibit myogenesis in C2C12 cells.

  • 精製度

    > 95 % SDS-PAGE.
    Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.

  • 発現系

    HEK 293 cells

  • タンパク質長

    Protein fragment

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      SGVFQLQLQE FINERGVLAS GRPCEPGCRT FFRVCLKHFQ AVVSPGPCTF GTVSTPVLGT NSFAVRDDSS GGGRNPLQLP FNFTWPGTFS LIIEAWHAPG DDLRPEALPP DALISKIAIQ GSLAVGQNWL LDEQTSTLTR LRYSYRVICS DNYYGDNCSR LCKKRNDHFG HYVCQPDGNL SCLPGWTGEY CQQPICLSGC HEQNGYCSKP AECLCRPGWQ GRLCNECIPH NGCRHGTCST PWQCTCDEGW GGLFCDQDLN YCTHHSPCKN GATCSNSGQR SYTCTCRPGY TGVDCELELS ECDSNPCRNG GSCKDQEDGY HCLCPPGYYG LHCEHSTLSC ADSPCFNGGS CRERNQGANY ACECPPNFTG SNCEKKVDRC TSNPCANGGQ CLNRGPSRMC RCRPGFTGTY CELHVSDCAR NPCAHGGTCH DLENGLMCTC PAGFSGRRCE VRTSIDACAS SPCFNRATCY TDLSTDTFVC NCPYGFVGSR CEFPVGLP

    • 領域

      27 to 524

特性

Our Abpromise guarantee covers the use of ab84081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態

    Lyophilized

  • 備考

    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Reconstituted ab84081 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC. Avoid repeated freeze/thaw cycles.

関連情報

  • 別名

    • AOS6
    • Delta 4
    • delta 4 precursor
    • Delta ligand 4
    • delta ligand 4 precursor
    • Delta like 4
    • Delta like 4 homolog
    • Delta like 4 protein
    • Delta like canonical Notch ligand 4
    • Delta like protein 4
    • Delta-like 4 (Drosophila)
    • Delta-like protein 4
    • Delta4
    • DLL 4
    • Dll4
    • DLL4_HUMAN
    • Drosophila Delta homolog 4
    • hdelta2
    • Homeobox protein DLL-4
    • MGC126344
    • Notch ligand delta 2
    • Notch ligand DLL4
    • Notch ligand DLL4 precursor
    • XDLL-4
    see all

  • 機能

    Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.

  • 組織特異性

    Expressed in vascular endothelium.

  • 配列類似性

    Contains 1 DSL domain.
    Contains 8 EGF-like domains.

  • ドメイン

    The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.

  • 翻訳後修飾

    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.

  • 細胞内局在

    Membrane.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab84081 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab84081 は 1 報の論文で使用されています。

  • Yu VW  et al. Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow. J Exp Med 212:759-74 (2015). PubMed: 25918341

レビューと Q&A

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Question

Dear whom it may concern,



I would like to ask about Dll4 protein product.

What is the mean of ‘active’ which is described in ab84081?

In other Dll4 product (ab108557), there is not this description.

I am glad if you can tell me the differences between these two products.

Thank you.

Read More
Answer

Thank you very much for contacting us with your question.

I've looked over both of these products, and they are both active DLL4 proteins. I'm not sure why ab108557 does not have "active" in its name, but I will recommend that this is updated.

Ab108557 has been shown to inhibit adipogenesis of 3T3L-1 cells and MSC's and also to induce Hes-1 in 3T3L-1 cells.Ab84081 has been used to inhibit myogenesis in C2C12 cells.

The major difference between these proteins is that ab84081 corresponds to amino acids 27-524 of human DLL4, while ab108557 is amino acids 1-529 and it is fused to a human IgG1 Fc fragment at the C terminus.

I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

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