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Recombinant human Dkk3 protein (ab186078)

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Key features and details

  • Expression system: CHO cells
  • Purity: > 98% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, HPLC, Functional Studies

製品の詳細

  • 製品名

    Recombinant human Dkk3 protein
    Dkk3 タンパク質・ペプチド 製品一覧

  • 生理活性

    Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 E1 cells. The expected ED50 for this effect is 2.0–4.0 ng/ml.

  • 精製度

    > 98 % SDS-PAGE.
    Purity is greater than 98% by SDS-PAGE gel and HPLC analyses.

  • 発現系

    CHO cells

  • アクセッション番号

    Q9UBP4

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      APAPTATSAP VKPGPALSYP QEEATLNEMF REVEELMEDT QHKLRSAVEE MEAEEAAAKA SSEVNLANLP PSYHNETNTD TKVGNNTIHV HREIHKITNN QTGQMVFSET VITSVGDEEG RRSHECIIDE DCGPSMYCQF ASFQYTCQPC RGQRMLCTRD SECCGDQLCV WGHCTKMATR GSNGTICDNQ RDCQPGLCCA FQRGLLFPVC TPLPVEGELC HDPASRLLDL ITWELEPDGA LDRCPCASGL LCQPHSHSLV YVCKPTFVGS RDQDGEILLP REVPDEYEVG SFMEEVRQEL EDLERSLTEE MALREPAAAA AALLGGEEI

    • 予測される分子量

      36 kDa

    • 領域

      22 to 350

特性

Our Abpromise guarantee covers the use of ab186078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    HPLC

    Functional Studies

  • 製品の状態

    Lyophilized

  • 備考

    Due to glycosylation, ab186078 migrates at an apparent molecular weight of approximately 39-49 kDa by SDS-PAGE analysis under non-reducing conditions.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    For lot specific reconstitution information please contact our Scientific Support Team.

関連情報

  • 別名

    • Dickkopf 3
    • Dickkopf 3 homolog
    • Dickkopf 3 homolog (Xenopus laevis)
    • Dickkopf homolog 3
    • Dickkopf related protein 3
    • Dickkopf related protein 3 Precursor
    • Dickkopf WNT signaling pathway inhibitor 3
    • Dickkopf, Xenopus, homolog of, 3
    • Dickkopf-3
    • Dickkopf-related protein 3
    • Dickkopf3
    • DKK 3
    • Dkk-3
    • DKK3
    • DKK3_HUMAN
    • hDkk 3
    • hDkk-3
    • hDkk3
    • Regulated in glioma
    • REIC
    • RIG
    • RIG-like 5-6
    • RIG-like 7-1
    see all

  • 機能

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

  • 組織特異性

    Highest expression in heart, brain, and spinal cord.

  • 配列類似性

    Belongs to the dickkopf family.

  • ドメイン

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

  • 翻訳後修飾

    N- and O-glycosylated.

  • 細胞内局在

    Secreted.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab186078 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab186078 は論文での使用が確認できていません。

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