Recombinant human DKK1 protein (ab82115)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant human DKK1 protein
DKK1 タンパク質・ペプチド 製品一覧 -
生理活性
Determined by its ability to inhibit mWnt3a-induced TCF/LEF-luciferase activity in reporter HEK293 cells.
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精製度
> 95 % SDS-PAGE.
Purity is greater than 97% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
HEK 293 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
TLNSVLNSNA IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab82115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.
pH: 7.50
Constituents: 0.877% Sodium chloride, 0.164% Sodium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成For lot specific reconstitution information please contact our Scientific Support Team. Centrifuge vial prior to opening. Initially reconstitute in water to 0.1-1.0 mg/ml. Do not vortex after reconstitution.
関連情報
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別名
- Dickkopf 1
- Dickkopf 1 homolog
- Dickkopf 1 like
see all -
機能
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
組織特異性
Placenta. -
配列類似性
Belongs to the dickkopf family. -
ドメイン
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab82115 は論文での使用が確認できていません。
