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AB152331

Recombinant Human DIO2 protein (GST tag N-Terminus)

Recombinant Human DIO2 protein (GST tag N-Terminus)

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Recombinant Human DIO2 protein (GST tag N-Terminus) is a Human Fragment protein, in the 166 to 265 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

別名を表示する

ITDI2, TXDI2, DIO2, Type II iodothyronine deiodinase, 5DII, DIOII, Type 2 DI, Type-II 5'-deiodinase

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SDS-PAGE - Recombinant Human DIO2 protein (GST tag N-Terminus) (AB152331)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DIO2 protein (GST tag N-Terminus) (AB152331)

12.5% SDS-PAGE analysis of ab152331 stained with Coomassie Blue.

Key facts

発現系

Wheat germ

タグ

GST tag N-Terminus

アプリケーション

ELISA, SDS-PAGE, WB

applications

生物活性

No

アクセッション番号

Q92813

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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組合せ製品

Primary Antibodies

AB77481

Anti-DIO2 antibody

Flow Cytometry (Intracellular) - Anti-DIO2 antibody (AB77481)

  • 5
  • 2
Primary Antibodies

AB109197

Anti-SAM68 antibody [EPR3232]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SAM68 antibody [EPR3232] (AB109197)

  • 0
  • 0
Primary Antibodies

AB233035

Anti-Dio3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dio3 antibody (AB233035)

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  • 1
Proteins & Peptides

AB153001

Recombinant Human KAT13D / CLOCK protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human KAT13D / CLOCK protein (GST tag N-Terminus) (AB153001)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"PSDGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQEVRHWLEKNFSKR","proteinLength":"Fragment","predictedMolecularWeight":"36.74 kDa","actualMolecularWeight":null,"aminoAcidEnd":265,"aminoAcidStart":166,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q92813","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Proprietary technique
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

'DIO2' also known as Type II iodothyronine deiodinase is an enzyme responsible for the conversion of thyroxine (T4) to the active hormone triiodothyronine (T3) by removal of an iodine atom. This transformation is important for regulating thyroid hormone activity. DIO2 has a molecular mass of approximately 58 kDa and is expressed predominantly in tissues such as the brain pituitary gland brown adipose tissue and skeletal muscle. It plays an integral role in maintaining intracellular T3 levels necessary for various physiological functions.
Biological function summary

DIO2 modulates local T3 availability which impacts metabolic rate thermogenesis and development. DIO2 is not part of a protein complex but interacts closely with thyroid hormone receptors that are activated by the produced T3. In adipocytes and myocytes DIO2-mediated T3 generation ensures the hormones' optimal action affecting processes such as energy expenditure and muscle function. This activity is critical in adapting to changing metabolic demands.

Pathways

DIO2 is integral to the thyroid hormone signaling pathway significantly influencing processes governed by thyroid hormone function. By converting T4 to T3 DIO2 interacts with thyroid hormone receptors and influences downstream effectors linked to metabolism and growth. DIO2 activity is regulated via the hypothalamic-pituitary-thyroid axis ensuring proper feedback loops and homeostasis. Additionally it is related to other deiodinases like DIO1 and DIO3 which also participate in thyroid hormone modification.

Alterations in DIO2 expression or activity have been linked to thyroid-related conditions such as hypothyroidism and Graves' disease. In hypothyroidism decreased DIO2 activity could lead to reduced local T3 levels affecting cellular metabolism. In Graves' disease overactive DIO2 may result in excessive T3 production exacerbating symptoms. DIO2's action further connects with thyroid hormone receptor beta (THRB) where abnormalities can contribute to resistance to thyroid hormones influencing disease progression and treatment outcomes.
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一般的な情報

機能

Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination (PubMed : 12586771, PubMed : 11108274, PubMed : 10403186, PubMed : 18821722). Catalyzes the deiodination of L-thyroxine (T4) to 3,5,3'-triiodothyronine (T3), 3,3',5'-triiodothyronine (rT3) to 3,3'-diiodothyronine (3,3'-T2) and 3',5'-diiodothyronine (3',5'-T2) to 3'-monoiodothyronine (3'-T1) via outer-ring deiodination (ORD) (PubMed : 12586771, PubMed : 11108274, PubMed : 10403186, PubMed : 18821722, PubMed : 18339710). Catalyzes the phenolic ring deiodinations of 3,3',5'-triiodothyronamine and 3',5'- diiodothyronamine (PubMed : 18339710).

配列の類似性

Belongs to the iodothyronine deiodinase family.

翻訳後修飾

Ubiquitinated by MARCHF6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33.

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製品プロトコール

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ターゲットの情報

Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination (PubMed : 12586771, PubMed : 11108274, PubMed : 10403186, PubMed : 18821722). Catalyzes the deiodination of L-thyroxine (T4) to 3,5,3'-triiodothyronine (T3), 3,3',5'-triiodothyronine (rT3) to 3,3'-diiodothyronine (3,3'-T2) and 3',5'-diiodothyronine (3',5'-T2) to 3'-monoiodothyronine (3'-T1) via outer-ring deiodination (ORD) (PubMed : 12586771, PubMed : 11108274, PubMed : 10403186, PubMed : 18821722, PubMed : 18339710). Catalyzes the phenolic ring deiodinations of 3,3',5'-triiodothyronamine and 3',5'- diiodothyronamine (PubMed : 18339710).
See full target information DIO2
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