Recombinant human Decorin protein (ab167743)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品の詳細
-
製品名
Recombinant human Decorin protein
Decorin タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its ability to modulate collagen fibrillogenesis. At 5 µg/mL, rhDecorin can significantly delay the rate of type I collagen fibrillogenesis. -
精製度
> 95 % SDS-PAGE.
ab167743 was lyophilized from 0.22 µm filtered solution. Purified by Immobilized metal affinity chromatography. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
GPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQ CSDLGLDKVPKDLPPDTTLLDLQN NKITEIKDGDFKNLKNLHALILVN NKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITK VRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITS IPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGS LANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCP PGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK -
予測される分子量
39 kDa including tags -
領域
17 to 359 -
タグ
His tag C-Terminus
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab167743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Functional Studies
-
製品の状態
Lyophilized -
備考
Measured by its ability to modulate collagen fibrillogenesis. At 5 µg/mL, rhDecorin can significantly delay the rate of type I collagen fibrillogenesis. -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 94% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成Reconstitute with sterile deionized water to a concentration of 600 µg/ml.
関連情報
-
別名
- Bone proteoglycan II
- CSCD
- DCN
see all -
機能
May affect the rate of fibrils formation. -
関連疾患
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. -
配列類似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
翻訳後修飾
The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
画像
-
SDS-PAGE - Recombinant human Decorin protein (ab167743)Human Decorin (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
-
SDS-PAGE - Recombinant human Decorin protein (ab167743)SDS-PAGE analysis of reduced ab167743 and staining overnight with Coomassie Blue.
Protein migrates as 45 kDa or higher due to different glycosylation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (1)
ab167743 は 1 報の論文で使用されています。
- Ozols M et al. Predicting Proteolysis in Complex Proteomes Using Deep Learning. Int J Mol Sci 22:N/A (2021). PubMed: 33803033
