Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品名Recombinant human Decorin protein
Decorin タンパク質・ペプチド 製品一覧
生理活性Measured by its ability to modulate collagen fibrillogenesis. At 5 µg/mL, rhDecorin can significantly delay the rate of type I collagen fibrillogenesis.
精製度> 95 % SDS-PAGE.
ab167743 was lyophilized from 0.22 µm filtered solution. Purified by Immobilized metal affinity chromatography.
エンドトキシン・レベル< 1.000 Eu/µg
発現系HEK 293 cells
タンパク質長Full length protein
配列GPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQ CSDLGLDKVPKDLPPDTTLLDLQN NKITEIKDGDFKNLKNLHALILVN NKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITK VRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITS IPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGS LANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCP PGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK
予測される分子量39 kDa including tags
領域17 to 359
タグHis tag C-Terminus
Our Abpromise guarantee covers the use of ab167743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
備考Measured by its ability to modulate collagen fibrillogenesis. At 5 µg/mL, rhDecorin can significantly delay the rate of type I collagen fibrillogenesis.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 94% PBS, 5% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成Reconstitute with sterile deionized water to a concentration of 600 µg/ml.
- Bone proteoglycan II
機能May affect the rate of fibrils formation.
関連疾患Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
配列類似性Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
翻訳後修飾The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
細胞内局在Secreted > extracellular space > extracellular matrix.
- Information by UniProt
Human Decorin (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
SDS-PAGE analysis of reduced ab167743 and staining overnight with Coomassie Blue.
Protein migrates as 45 kDa or higher due to different glycosylation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab167743 は 1 報の論文で使用されています。
- Ozols M et al. Predicting Proteolysis in Complex Proteomes Using Deep Learning. Int J Mol Sci 22:N/A (2021). PubMed: 33803033