Recombinant Human DC-SIGNR protein (Fc Chimera) (ab83920)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human DC-SIGNR protein (Fc Chimera)
DC-SIGNR タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
Theoretical Sequence: HGTELPSPPSKLQVSKVPSSLSQEQSEQDAIYQNLTQLKAAVGELSEK SKLQEIYQELTQLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQ EIYQELTRLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQ ELTELKAAVGELPEKSKLQEIYQELTQLKAAVGELPDQSKQQQIYQELTD LKTAFERLCRHCPKDWTFFQGNCYFMSNSQRNWHDSVTACQEVRAQLVVI KTAEEQNFLQLQTSRSNRFSWMGLSDLNQEGTWQWVDGSPLSPSFQRYWN SGEPNNSGNEDCAEFSGSGWNDNRCDVDNYWICKKPAACFRDGIPKVDKK VEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVV DVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWL NGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVS LTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDK SRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
タグ
Fc tag C-Terminus -
配列の追加情報
Fused with the Fc region of Human IgG1 at the C-terminus.
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特性
Our Abpromise guarantee covers the use of ab83920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C.
Constituents: 10% Trehalose, 1% Human serum albumin
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再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
関連情報
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別名
- C type lectin domain family 4, member M
- C-type lectin domain family 4 member M
- CD209 antigen like
see all -
機能
Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus. -
組織特異性
Predominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells. -
配列類似性
Contains 1 C-type lectin domain. -
ドメイン
The tandem repeat domain, also called neck domain, mediates oligomerization. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human DC-SIGNR protein (Fc Chimera) (ab83920)Lane 1 – MW markers; Lane 2 – ab83920; Lane 3 – ab83920 treated with PNGase F to remove potential N-linked glycans. 10 μg protein loaded per lane. Deep Purple stained.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Band in lane 3 at 35 kDa is PNGase F protein. -
SDS-PAGE - Recombinant Human DC-SIGNR protein (Fc Chimera) (ab83920)A sample of ab83920 without carrier protein was reduced and alkylated. 40 μg protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train (Deep Purple™ stained) indicates presence of multiple glycoforms of ab83920. Spots within the spot train were cut from the gel and identified by protein mass fingerprinting as DC-SIGNR (Fc Chimera).
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Functional Studies - Recombinant Human DC-SIGNR protein (Fc Chimera) (ab83920)Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.
The triangle indicates theoretical pI and MW.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab83920 は論文での使用が確認できていません。