AB83920

Recombinant Human DC-SIGNR protein (Fc Chimera)

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Recombinant Human DC-SIGNR protein (Fc Chimera) is a Human Fragment protein, expressed in HEK 293 cells, with >95%, suitable for FuncS, SDS-PAGE.

別名を表示する

CD299, CD209L, CD209L1, CLEC4M, C-type lectin domain family 4 member M, CD209 antigen-like protein 1, DC-SIGN-related protein, Dendritic cell-specific ICAM-3-grabbing non-integrin 2, Liver/lymph node-specific ICAM-3-grabbing non-integrin, DC-SIGNR, DC-SIGN2, L-SIGN

3 Images

Functional Studies - Recombinant Human DC-SIGNR protein (Fc Chimera) (AB83920)

FuncS

Unknown

Functional Studies - Recombinant Human DC-SIGNR protein (Fc Chimera) (AB83920)

Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.

The triangle indicates theoretical pI and MW.

SDS-PAGE - Recombinant Human DC-SIGNR protein (Fc Chimera) (AB83920)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DC-SIGNR protein (Fc Chimera) (AB83920)

Lane 1 – MW markers; Lane 2 – ab83920; Lane 3 – ab83920 treated with PNGase F to remove potential N-linked glycans. 10 μg protein loaded per lane. Deep Purple stained.

Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Band in lane 3 at 35 kDa is PNGase F protein.

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DC-SIGNR protein (Fc Chimera) (AB83920)

A sample of ab83920 without carrier protein was reduced and alkylated. 40 μg protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train (Deep Purple™ stained) indicates presence of multiple glycoforms of ab83920. Spots within the spot train were cut from the gel and identified by protein mass fingerprinting as DC-SIGNR (Fc Chimera).

Key facts

精製度

>95% SDS-PAGE

発現系

HEK 293 cells

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

アクセッション番号

Q9H2X3

アニマルフリー

キャリアフリー

種

Human

再構成

Reconstitute in 0.5 mL of PBS

バッファー組成

Constituents: 10% Trehalose, 1% Human serum albumin

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Molecular Mass: DC-SIGNR-Fc Chimera migrates as a broad band between 70 and 85 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified DC-SIGNR-Fc Chimera that has a predicted molecular mass of 65.6 kDa.pI: DC-SIGNR-Fc Chimera separates into a number of glycoforms on 2D PAGE due to post-translational modifications, in particular glycosylation. The pI range is between 5.4 and 6.5. This compares with the unmodified DC-SIGNR-Fc Chimera that has a predicted pI of 5.70." } } }

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配列情報

[{"sequence":"HGTELPSPPSKLQVSKVPSSLSQEQSEQDAIYQNLTQLKAAVGELSEKSKLQEIYQELTQLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTELKAAVGELPEKSKLQEIYQELTQLKAAVGELPDQSKQQQIYQELTDLKTAFERLCRHCPKDWTFFQGNCYFMSNSQRNWHDSVTACQEVRAQLVVIKTAEEQNFLQLQTSRSNRFSWMGLSDLNQEGTWQWVDGSPLSPSFQRYWNSGEPNNSGNEDCAEFSGSGWNDNRCDVDNYWICKKPAACFRDGIPKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9H2X3","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

False

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates.. (Microbial infection) Acts as an attachment receptor for Ebolavirus.. (Microbial infection) Acts as an attachment receptor for Hepatitis C virus.. (Microbial infection) Acts as an attachment receptor for HIV-1.. (Microbial infection) Acts as an attachment receptor for Human coronavirus 229E.. (Microbial infection) Acts as an attachment receptor for Human cytomegalovirus/HHV-5.. (Microbial infection) Acts as an attachment receptor for Influenzavirus.. (Microbial infection) Acts as an attachment receptor for SARS-CoV.. (Microbial infection) Acts as an attachment receptor for West-nile virus.. (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus.. (Microbial infection) Acts as an attachment receptor for Marburg virus glycoprotein.. (Microbial infection) Recognition of M.bovis by dendritic cells may occur partially via this molecule.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information CLEC4M

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Recombinant Human DC-SIGNR protein (Fc Chimera)