AB264453

Recombinant Human Cytokeratin 14 protein (His tag N-Terminus)

Name: Recombinant Human Cytokeratin 14 protein (His tag) (ab264453) | Abcam
Brand: Abcam Limited
SKU: AB264453
Price: 108000 JPY
Availability: InStock

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Recombinant Human Cytokeratin 14 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 472 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

別名を表示する

Cytokeratin-14, Keratin-14, CK-14, K14, KRT14

1 Images

SDS-PAGE - Recombinant Human Cytokeratin 14 protein (His tag N-Terminus) (AB264453)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Cytokeratin 14 protein (His tag N-Terminus) (AB264453)

SDS-PAGE analysis of ab264453 (3 μg) under reducing conditions. Coomassie Blue staining. 15% SDS-PAGE gel.

Key facts

精製度

>85% SDS-PAGE

Purified by using conventional chromatography.

発現系

Escherichia coli

アプリケーション

SDS-PAGE

applications

生物活性

アクセッション番号

P02533

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSSRFSSGGAYGLGGGYGGGFSSSSSSFGSGFGGGYGGGLGTGLGGGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPAEIKDYSPYFKTIEDLRNKILTATVDNANVLLQIDNARLAADDFRTKYETELNLRMSVEADINGLRRVLDELTLARADLEMQIESLKEELAYLKKNHEEEMNALRGQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN","proteinLength":"Full Length","predictedMolecularWeight":"53.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":472,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P02533","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

製品の状態

Liquid

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 14 also known as CK14 keratin 14 or KRT14 is a type I keratin protein with a molecular mass of approximately 50 kDa. It forms intermediate filaments in epithelial cells primarily in the basal layer of stratified epithelium and is part of the cytoskeleton. Cytokeratin 14 expression appears in tissues such as the epidermis tongue and esophagus where it contributes to the structural integrity and mechanical stability of cells. Laboratories often utilize antibodies like anti-K14 and labels such as Alexa Fluor 555 or Alexa Fluor 647 to identify cytokeratin 14 in research settings.

Biological function summary

This protein significantly contributes to the maintenance and regeneration of epithelial tissues by forming a network of filaments with keratin 5. KRT14 pairs with keratin 5 to create a keratin intermediate filament complex which plays a structural role in the resilience and elasticity of epithelial tissues. This partnership is essential for the assembly of the cytoskeleton and supports the protective barrier function of the skin and related tissues.

Pathways

Cytokeratin 14 engages in pivotal cellular processes like keratinization and wound healing. The protein is actively involved in signal transduction pathways such as the epithelial cell differentiation pathway. Within these pathways KRT14 collaborates with KRT5 and other structural proteins to regulate cell proliferation and differentiation reflecting its importance in skin health and function.

Cytokeratin 14 associates with specific genetic conditions including epidermolysis bullosa simplex (EBS) and squamous cell carcinoma (SCC). In EBS mutations in the KRT14 gene lead to skin fragility. In SCC altered expression of KRT14 and related proteins such as keratin 5 can influence tumor progression and invasion. These associations highlight the critical role of KRT14 in both normal and pathological epithelial function.

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一般的な情報

機能

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

配列の類似性

Belongs to the intermediate filament family.

翻訳後修飾

A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Keratin, type I cytoskeletal 14

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Recombinant Human Cytokeratin 14 protein (His tag N-Terminus)