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AB119468

Recombinant Human CYB5R3 protein (His tag N-Terminus)

Recombinant Human CYB5R3 protein (His tag N-Terminus)

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Recombinant Human CYB5R3 protein (His tag N-Terminus) is a Human Full Length protein, in the 27 to 301 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

DIA1, CYB5R3, NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1

1 Images
SDS-PAGE - Recombinant Human CYB5R3 protein (His tag N-Terminus) (AB119468)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CYB5R3 protein (His tag N-Terminus) (AB119468)

15% SDS-PAGE analysis of ab119468 (3μg)

Key facts

精製度

>95% SDS-PAGE

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

Mass Spec, SDS-PAGE

applications

生物活性

No

アクセッション番号

P00387

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF","proteinLength":"Full Length","predictedMolecularWeight":"34 kDa","actualMolecularWeight":null,"aminoAcidEnd":301,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00387","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein CYB5R3 also known as Cytochrome b5 reductase 3 has a molecular mass of approximately 34 kDa. This enzyme is located in various cellular compartments including the outer mitochondrial membrane and the endoplasmic reticulum. CYB5R3 plays a role in electron transfer by reducing cytochrome b5 using NADH or NADPH as electron donors. This reduction is important for various biosynthesis reactions including fatty acid desaturation and elongation as well as steroid biosynthesis.
Biological function summary

CYB5R3 contributes to the electron transport chain by facilitating the transfer of reducing equivalents to other systems. This enzyme efficiently elevates the electron flow when functioning within a larger enzymatic complex. CYB5R3 is necessary for maintaining redox balance in cells and supports the synthesis of important biomolecules like cholesterol and lipids due to its involvement in these processes.

Pathways

CYB5R3 integrates into the fatty acid metabolism and steroid biosynthesis pathways. It effectively partners with cytochrome b5 and other reductases to enable efficient electron flux within these pathways. CYB5R3's activity supports metabolic cooperation with other enzymes such as fatty acid desaturase promoting the production and transformation of essential fatty acids.

Mutations or malfunctions in CYB5R3 lead to conditions such as methemoglobinemia and insulin resistance. Methemoglobinemia results from altered oxygen delivery in the blood due to improper reduction of methemoglobin. In this context the reduced function of CYB5R3 affects the ability of related proteins like hemoglobin to bind oxygen efficiently. Insulin resistance involves metabolic alterations where CYB5R3 acts in coordination with proteins associated with lipid metabolism influencing energy homeostasis and insulin action.
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製品の性状

製品の状態

Liquid

補足情報

ab119468 was purified using conventional chromatography.

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一般的な情報

機能

Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.

配列の類似性

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

細胞内局在性

Mitochondrion outer membrane

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製品プロトコール

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ターゲットの情報

Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
See full target information CYB5R3
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組合せ製品

Primary Antibodies

AB133247

Anti-CYB5R3 antibody [EPR3868]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R3 antibody [EPR3868] (AB133247)

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