Recombinant Human CXCL4 protein (Active) (ab283898)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, MS, HPLC, Biological Activity
製品の詳細
-
製品名
Recombinant Human CXCL4 protein (Active)
PF4 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the ability to inhibit FGF-basic dependent proliferation of HUVEC. ED50 is ≤ 10.5 ug/ml, corresponding to a specific activity of 95.2 units/mg.
-
精製度
>= 95 % HPLC.
SDS PAGE>=95% Purity -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
-
生物種
Human -
配列
EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRK ICLDLQAPLYKKIIKKLLES -
予測される分子量
78 kDa -
実際の分子量
78 kDa -
分子量情報
Predicted MW is 7826.2 Da (+/- 10 Da by ESI-TOF). Observed MW is 7826.03 Da. -
領域
32 to 101 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide and pro-peptide.
-
特性
Our Abpromise guarantee covers the use of ab283898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Mass Spectrometry
HPLC
Biological Activity
-
製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate bufferred saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
関連情報
-
別名
- C-X-C motif chemokine 4
- Chemokine (C X C motif) ligand 4
- Chemokine (CXC motif) ligand 4
see all -
機能
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
Binds non-covalently to a proteoglycan molecule. -
細胞内局在
Secreted. - Information by UniProt
画像
-
Functional Studies - Recombinant Human CXCL4 protein (ab283898)
Fully biologically active determined by the ability to inhibit FGF-basic dependent proliferation of HUVEC. ED50 is ≤ 10.5 ug/ml, corresponding to a specific activity of 95.2 units/mg.
Cell based assay testing is performed on the first lot of the protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3431381-1.
-
Mass Spectrometry - Recombinant Human CXCL4 protein (ab283898)Mass determination by ESI-TOF.
Predicted MW is 7826.2 (+/- 10 Da by ESI-TOF). Observed MW is 7826.03 Da.
-
HPLC - Recombinant Human CXCL4 protein (ab283898)HPLC analysis of ab283898.
-
SDS-PAGE - Recombinant Human CXCL4 protein (ab283898)SDS-PAGE analysis of ab283898.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab283898 は論文での使用が確認できていません。
