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AB283898

Recombinant Human CXCL4 protein (Active)

Recombinant Human CXCL4 protein (Active)

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Recombinant Human CXCL4 protein (Active) is a Human Full Length protein, in the 32 to 101 aa range, expressed in HEK 293 cells, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, FuncS.

別名を表示する

CXCL4, SCYB4, PF4, Platelet factor 4, PF-4, C-X-C motif chemokine 4, Iroplact, Oncostatin-A

4 Images
Mass Spectrometry - Recombinant Human CXCL4 protein (Active) (AB283898)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human CXCL4 protein (Active) (AB283898)

Mass determination by ESI-TOF. Predicted MW is 7826.2 (+/- 10 Da by ESI-TOF). Observed MW is 7826.03 Da.

Biochemical assay - Recombinant Human CXCL4 protein (Active) (AB283898)
  • Biochemical assay

Supplier Data

Biochemical assay - Recombinant Human CXCL4 protein (Active) (AB283898)

Fully biologically active determined by the ability to inhibit FGF-basic dependent proliferation of HUVEC. ED50<= 10.5 ug/ml, corresponding to a specific activity of 95.2 units/mg.

SDS-PAGE - Recombinant Human CXCL4 protein (Active) (AB283898)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CXCL4 protein (Active) (AB283898)

SDS-PAGE analysis of ab283898.

HPLC - Recombinant Human CXCL4 protein (Active) (AB283898)
  • HPLC

Supplier Data

HPLC - Recombinant Human CXCL4 protein (Active) (AB283898)

HPLC analysis of ab283898.

Key facts

精製度

undefined HPLC

SDS PAGE>=95% Purity

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

タグ

Tag free

アプリケーション

Mass Spec, HPLC, SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

Fully biologically active determined by the ability to inhibit FGF-basic dependent proliferation of HUVEC. ED50 is ≤ 10.5 ug/ml, corresponding to a specific activity of 95.2 units/mg.

アクセッション番号

P02776

アニマルフリー

Yes

キャリアフリー

No

種

Human

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

  • 0
  • 0
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB201279

Mouse E-Selectin ELISA Kit (CD62E)

Recombinant|SimpleStep

Sandwich ELISA - Mouse E-Selectin ELISA Kit (CD62E) (AB201279)

  • 0
  • 0
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
Assay Kits

AB223591

Human VCAM1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human VCAM1 ELISA Kit (AB223591)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES","proteinLength":"Full Length","predictedMolecularWeight":"78.26 kDa","actualMolecularWeight":"78.26 kDa","aminoAcidEnd":101,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P02776","tags":[]}]
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出荷温度及び保存条件

製品の状態
Lyophilized
出荷温度
Ambient - Can Ship with Ice
短期保存温度
Ambient
長期保存温度
Ambient
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein Platelet Factor 4 (PF4) also known as CXCL4 is a member of the chemokine (C-X-C motif) ligand family. This small protein has a molecular weight of approximately 7.8 kDa. PF4 is mainly expressed in megakaryocytes and stored in the alpha granules of platelets. During platelet activation PF4 releases into the bloodstream where it interacts with other proteins and cell types. Researchers measure PF4 levels with methods such as PF4 ELISA to study its expression and functions under various physiological and pathological conditions.
Biological function summary

PF4 plays an important role in modulating the immune response and coagulation processes. PF4 is not part of a stable protein complex but can interact dynamically with heparin on the surface of endothelial cells and glycosaminoglycans. It inhibits the action of heparin promoting clot formation and angiogenesis. PF4 also attracts and activates different types of leukocytes and can regulate inflammatory processes influencing how the immune system responds to injury.

Pathways

PF4 significantly interacts with both the coagulation and inflammation pathways. In the coagulation pathway PF4 acts to neutralize heparin-like molecules thereby enhancing thrombosis. It works closely with proteins such as thrombin and fibrinogen contributing to the blood clot cascade. Within the inflammatory pathway PF4's chemokine activity helps direct leukocytes to sites of tissue damage or infection linking it to cytokines and other chemokines that mediate immune responses.

Researchers associate PF4 with conditions such as thrombosis and autoimmune pathologies. In thrombosis the overexpression of PF4 can lead to excessive clot formation pointing to its interaction with proteins like thrombin. PF4 also plays a role in heparin-induced thrombocytopenia (HIT) a disorder where the immune system mistakenly produces antibodies against heparin-PF4 complexes further indicating its direct involvement with immune and coagulation pathways.
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一般的な情報

機能

Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation (PubMed : 29930254, PubMed : 9531587). Acts via different functional receptors including CCR1, CXCR3A or CXCR3B (PubMed : 18174362, PubMed : 29930254). Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling (PubMed : 18174362, PubMed : 24469069). Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence (PubMed : 9531587). Chemotactic for neutrophils and monocytes via CCR1 (PubMed : 29930254). Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis (PubMed : 35701499). In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition (PubMed : 34986347). Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity (PubMed : 9395524).

配列の類似性

Belongs to the intercrine alpha (chemokine CxC) family.

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製品プロトコール

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ターゲットの情報

Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation (PubMed : 29930254, PubMed : 9531587). Acts via different functional receptors including CCR1, CXCR3A or CXCR3B (PubMed : 18174362, PubMed : 29930254). Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling (PubMed : 18174362, PubMed : 24469069). Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence (PubMed : 9531587). Chemotactic for neutrophils and monocytes via CCR1 (PubMed : 29930254). Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis (PubMed : 35701499). In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition (PubMed : 34986347). Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity (PubMed : 9395524).
See full target information PF4
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