Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
製品の詳細
-
製品名
Recombinant human Cripto1/CRIPTO protein (Fc Chimera)
Cripto1/CRIPTO タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
Theoretical sequence: LGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPP MGIQHSKEL NRTCCLNGGTCMLGSFCACPPSFYGRNCEHDVRKENCGS VPHDTWLPK KCSLCKCWHGQLRCFPQAFLPGCDGLVMDEHLVASRTPE LPPSGSSNT KVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPK DTLMISRTP EVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQY NSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPR EPQVYTLPP SRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYK TTPPVLDSD GSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGK -
領域
31 to 169 -
配列の追加情報
Encodes the signal peptide and extracellular domain of human Cripto-1 (aa 1-169) was fused to the Fc region of human IgG1 (aa 90-330). The chimeric protein was expressed in modified human 293 cells.
-
特性
Our Abpromise guarantee covers the use of ab84062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
-
製品の状態
Lyophilized -
備考
200 ng/ml of this Chimera induces ERK1 and ERK2 phosphorylation in human umbilical vein endothelial (HUVEC) cells.This product was previously labelled as Cripto1
This product was previously labelled as Cripto1
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 1% Human serum albumin, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
関連情報
-
別名
- CR
- CRGF
- cripto
see all -
機能
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. -
組織特異性
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. -
配列類似性
Contains 1 EGF-like domain. -
細胞内局在
Cell membrane. - Information by UniProt
画像
-
SDS-PAGE - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062)Lane 1 – MW markers; Lane 2 – ab84062; Lane 3 – ab84062 treated with PNGase F to remove potential N linked glycans; Lane 4 – ab84062 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.
Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. -
SDS-PAGE - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062)A sample of ab84062 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4 20% Tris-HCl 2D gel. Approximately 40 μg of protein was loaded; Gel was stained using Deep Purple™. The spot train indicates the presence of multiple glycoforms. Spots within the spot train were cut from the gel and identified as Cripto1/CRIPTO (Fc Chimera) by protein mass fingerprinting.
-
Functional Studies - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062)Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.
The triangle indicates theoretical pI and MW of the protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab84062 は論文での使用が確認できていません。
