Recombinant human Cripto1/CRIPTO protein (ab84064)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
製品の詳細
-
製品名
Recombinant human Cripto1/CRIPTO protein
Cripto1/CRIPTO タンパク質・ペプチド 製品一覧 -
生理活性
ab84064 has been shown to stimulate MAPK phosphorylation in HUVEC cells. 200ng/ml is sufficient to stimulate phosphorylation. -
精製度
> 95 % SDS-PAGE. -
発現系
HEK 293 cells -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
Theoretical sequence: LGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPP MGIQHSKELN RTCCLNGGTCMLGSFCACPPSFYGRNCEHDVRKENCGS VPHDTWLPKKC SLCKCWHGQLRCFPQAFLPGCDGLVMDEHLVASRTPE LPPS -
領域
31 to 169
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab84064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
-
製品の状態
Lyophilized -
備考
ab84064 has been shown to stimulate MAPK phosphorylation in HUVEC cells. 200ng/ml is sufficient to stimulate phosphorylation.This product was previously labelled as Cripto1
This product was previously labelled as Cripto1
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 1% Human serum albumin, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term sto rage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
関連情報
-
別名
- CR
- CRGF
- cripto
see all -
機能
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. -
組織特異性
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. -
配列類似性
Contains 1 EGF-like domain. -
細胞内局在
Cell membrane. - Information by UniProt
画像
-
SDS-PAGE - Recombinant human Cripto1/CRIPTO protein (ab84064)Lane 1- MW markers; Lane 2- ab84064 ; Lane 3- ab84064 treated with PNGase F to remove potential N-linked glycans; Lane 4- ab84064 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.
A drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
O-fucosylation at Thr-88 has been confirmed.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab84064 は論文での使用が確認できていません。
