Recombinant human Chk1 protein (ab60762)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Chk1 protein
Chk1 タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
Purity: >95% as determined by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab60762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- C85740
- Cell cycle checkpoint kinase
- Checkpoint , S. pombe, homolog of, 1
see all - Information by UniProt
画像
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Functional Studies - Recombinant human Chk1 protein (ab60762)The specific activity of Chk1 (ab60762) was determined to be 228 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human Chk1 protein (ab60762)SDS PAGE analysis of ab60762 -
Functional Studies - Recombinant human Chk1 protein (ab60762)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human Chk1 protein (ab60762)ab60762 on SDS-PAGE, MW ~82kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab60762 は 1 報の論文で使用されています。
- Jaiswal RK et al. CaMKK2 and CHK1 phosphorylate human STN1 in response to replication stress to protect stalled forks from aberrant resection. Nat Commun 14:7882 (2023). PubMed: 38036565