Recombinant Human CENPH protein (denatured) (ab113134)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human CENPH protein (denatured) -
精製度
> 90 % SDS-PAGE.
ab113134 was denatured using detergent during a conventional chromatography purification process. -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MGSSHHHHHHSSGLVPRGSHMLNKLIMKSQQESWDLEEKLLDI RKKRL QLKQASESKLLEIQTEKNKQKIDLDSMENSERIKIIRQNLQMEIK ITT VIQHVFQNLILGSKVNWAEDPALKEIVLQLEKNVDMM -
予測される分子量
16 kDa including tags -
領域
136 to 247 -
タグ
His tag N-Terminus
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-
製品の詳細
Recombinant Human CENPH protein
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab113134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)
関連情報
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別名
- CENP H
- CENP-H
- CENPH
see all -
機能
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. -
配列類似性
Belongs to the centromere protein H family. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human CENPH protein (denatured) (ab113134)15% SDS-PAGE analysis of 3 µg ab113134.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113134 は論文での使用が確認できていません。