Recombinant Human Cdk9 protein (ab85603)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE
製品の詳細
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製品名
Recombinant Human Cdk9 protein -
精製度
> 85 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
タグ
GST tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab85603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
関連情報
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別名
- C-2K
- CDC2 related kinase
- CDC2L4
see all -
機能
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Nucleus. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human Cdk9 protein (ab85603)SDS-PAGE showing ab85603 at approximately 68kDa.
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Sandwich ELISA - Recombinant Human Cdk9 protein (ab85603)Standard Curve for Cdk9 (Analyte: Cdk9 protein (Tagged) (ab85603)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to Cdk9 (ab76873) at 5µg/ml and Detector Antibody Rabbit polyclonal to Cdk9 (ab38840) at 0.5µg/ml.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab85603 は 1 報の論文で使用されています。
- Ma X et al. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. Elife 8:N/A (2019). PubMed: 30652970