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Recombinant human CDK5 + p25 protein (ab60761)

Reviews (1) Submit a question References (2)
Functional Studies - Recombinant human CDK5 + p25 protein (ab60761)
  • SDS-PAGE - Recombinant human CDK5 + p25 protein (ab60761)
  • Functional Studies - Recombinant human CDK5 + p25 protein (ab60761)
  • SDS-PAGE - Recombinant human CDK5 + p25 protein (ab60761)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: WB, Functional Studies, SDS-PAGE

製品の詳細

特性

Our Abpromise guarantee covers the use of ab60761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    Functional Studies

    SDS-PAGE

  • 製品の状態

    Liquid

  • 備考

    ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

画像

  • Functional Studies - Recombinant human CDK5 + p25 protein (ab60761)
    Functional Studies - Recombinant human CDK5 + p25 protein (ab60761)
    The specific activity of Cdk5 + Cyclin-dependent kinase 5 activator 1, p25 (ab60761) was determined to be 645 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human CDK5 + p25 protein (ab60761)
    SDS-PAGE - Recombinant human CDK5 + p25 protein (ab60761)
    SDS PAGE analysis of ab60761
  • Functional Studies - Recombinant human CDK5 + p25 protein (ab60761)
    Functional Studies - Recombinant human CDK5 + p25 protein (ab60761)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human CDK5 + p25 protein (ab60761)
    SDS-PAGE - Recombinant human CDK5 + p25 protein (ab60761)
    ab60761 on SDS-PAGE, p35 MW 59kDa and p25 49kDa.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab60761 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab60761 は 2 報の論文で使用されています。

  • Nakamura T  et al. Noncanonical transnitrosylation network contributes to synapse loss in Alzheimer's disease. Science 371:N/A (2021). PubMed: 33273062
  • Jin X  et al. PES1 promotes BET inhibitors resistance and cells proliferation through increasing c-Myc expression in pancreatic cancer. J Exp Clin Cancer Res 38:463 (2019). PubMed: 31718704

レビューと Q&A

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Western blot Abreview for p35 + p25 protein (Tagged)

 Good
Abreviews
Application
Western blot
Review text: The blot image provided in this review has mouse Dorsal Striatum loaded at 70ug in lanes 2-7 and the P35/P25-GST lysate in .5, 1 and 3 ug in lanes 8-10. The .5ug amount appears to be the least necessary to produce both the p35-GST and p25-GST bands. The p25-GST band is much stronger than the p35-GST band. A goat polyclonal anti-GST antibody was used from GE Health sciences. Therefore, lanes 8-10 have a yellow band because of co-labeling of p35/p25 and the GST tag. There was some nonspecific binding with the p35/p25-GST lysate due to such a high amount being used. These bands are found mainly below the p25 band. There was also nonspecific binding with the anti-GST antibody within the p35/p25-GST lysate but not the Dorsal Striatum lysates. Only p35 appears in the Dorsal Striatum lanes because the 10% gel was not able to resolve p25 because it is too light. 12.5% gels have been used to resolve both p35 and p25 in previous studies, but this percent gel would not have been able to resolve the GST tagged proteins. A gradient gel would be necessary.
Sample: Mouse Cell lysate - whole cell (Brain)
Loading amount: 70 µg
Specification: Brain
Gel Running Conditions: Reduced Denaturing (10% gel)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details
Incubation time: 18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk

Primary antibody (in addition to 'p35 + p25 protein (Tagged)')
Name: Non-Abcam antibody was used: CST anti-p35/p25
Host species: Rabbit
Clonality: Monyclonal
Conjugation: Other
Dilution: 1/1000

Secondary antibody
Name: Non-Abcam antibody was used: Licor 700
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX

Detection
Detection method: Licor
Exposure: 1 hour(s), 0 minute(s) and 0 second(s)
Bands: Specific: 59 and 49 kDa Non-specific: several below 40 kDa
Positive control: The p35/p25-GST lysate served as a positive control
Negative control: None
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Apr 08 2011

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