Recombinant human CD80 protein (Active) (ab173993)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: ELISA, HPLC, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human CD80 protein (Active)
CD80 タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, His Tag, Fc Tag (ab246118) with a linear range of 0.02-1.25 μg/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 2 μg/mL (100 μL/well) can bind Recombinant human CTLA4 protein (ab167727) with a linear range of 0.5-4 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 (biotinylated) protein (ab207136) with a linear range of 0.6-10 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Mouse CD28, His, Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind ab173993 with a linear range of 0.2-2 ng/mL.
Measured by its binding ability in a SPR assay. Immobilized Human CD28, on CM5 Chip can bind ab173993 with an affinity constant of 2.01 μM.
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag with a linear range of 0.1-2 ng/mL (QC tested).
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精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIW PEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEV TLSVKADFPTPSISDFEIPT SNIRRIICSTSGGFPEPHLSWLENGEEL NAINTTVSQDPETELYAVSSKLDFNMTTNHSF MCLIKYGHLRVNQTFN WNTTKQEHFPDN -
予測される分子量
50 kDa including tags -
分子量情報
The protein migrates as 65-90 kDa under reducing condition due to glycosylation. -
領域
35 to 242 -
タグ
Fc tag C-Terminus -
配列の追加情報
(NP_005182.1) ab173993 is fused with Fc fragment of Human IgG1 at the C terminus.
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特性
Our Abpromise guarantee covers the use of ab173993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
HPLC
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
B7-1 and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant co-stimulatory pathways that regulate T and Bcell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B71 and B72 with a 20 100 fold higher affinity than CD28 and is involved in the downregulation of the immune response.
B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Please see notes section.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 10% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
関連情報
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別名
- Activation B7-1 antigen
- B lymphocyte activation antigen B7
- B7
see all -
機能
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
組織特異性
Expressed on activated B-cells, macrophages and dendritic cells. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
画像
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SDS-PAGE - Recombinant human CD80 protein (ab173993)
Reduced SDS-PAGE analysis of ab173993, stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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ELISA - Recombinant human CD80 protein (ab173993)Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 (biotinylated) protein (ab207136) with a linear range of 0.6-10 ng/mL.
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Functional Studies - Recombinant human CD80 protein (ab173993)FACS assay shows that ab173993 can bind to 293T cells overexpressing human CTLA4 protein. The concentration of ab173993 is 1 μg/mL.
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Functional Studies - Recombinant human CD80 protein (ab173993)Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, His Tag, Fc Tag (ab246118) with a linear range of 0.02-1.25 μg/mL
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Functional Studies - Recombinant human CD80 protein (ab173993)Immobilized Human CD28, on CM5 Chip can bind ab173993 with an affinity constant of 2.01 μM as determined in a SPR assay.
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ELISA - Recombinant human CD80 protein (ab173993)Immobilized ab173993 at 2 μg/mL (100 μL/well) can bind Recombinant human CTLA4 protein (ab167727) with a linear range of 0.5-4 ng/mL.
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ELISA - Recombinant human CD80 protein (ab173993)Immobilized Biotinylated Mouse CD28, His, Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind ab173993 with a linear range of 0.2-2 ng/mL.
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ELISA - Recombinant human CD80 protein (Active) (ab173993)ab173993 at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag with a linear range of 0.1-2 ng/mL (QC tested).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab173993 は論文での使用が確認できていません。
