Recombinant Human CD62E protein (ab167744)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant Human CD62E protein
CD62E タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
ab167744 was lyophilized from 0.22 µm filtered solution. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
WSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYW IGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKD VGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSG LKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMET MQCMSSGEWSAPIPACNVVECDAVTNPANGFVECFQNPGSFPWNTTCTFD CEEGFELMGAQSLQCTSSGNWDNEKPTCKAVTCRAVRQPQNGSVRCSHSP AGEFTFKSSCNFTCEEGFMLQGPAQVECTTQGQWTQQIPVCEAFQCTA LSNPERGYMNCLPSASGSFRYGSSCEFSCEQGFVLKGSKRLQCGPTGEWD NEKPTCEAVRCDAVHQPPKGLVRCAHSPIGEFTYKSSCAFSCEEGFELHG STQLECTSQGQWTEEVPSCQVVKCSSLAVPGKINMSCSGEPVFGTVCKFA CPEGWTLNGSAARTCGATGHWSGLLPTCEAPTESNIP -
予測される分子量
60 kDa including tags -
領域
22 to 556 -
タグ
His tag C-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab167744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
Measured by the ability of the immobilized protein to support the adhesion of U937 Human histiocytic lymphoma cells. When 5 x 104cells/well are added to Human CD62E coated plates (2 µg/mL with 100 µL/well), >80% cells will adhere after 1 hour incubation at 37°C. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 94% PBS, 5% Trehalose -
再構成It is strongly recommended to reconstitute the lyophilized ab167744 with 2000 µl sterile deionized water to a final concentration of 100 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
関連情報
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別名
- CD26E
- CD62 antigen-like family member E
- CD62E
see all -
機能
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. -
配列類似性
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains. -
細胞内局在
Membrane. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human CD62E protein (ab167744)
SDS PAGE analysis of ab167744 under reducing conditions with overnight staining in Coomassie Blue
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SDS-PAGE - Recombinant human CD62E protein (ab167744)SDS-PAGE analysis of reduced ab167744 and staining overnight with Coomassie Blue. Protein migrates as 110 kDa in reduced SDS-PAGE resulting from glycosylation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab167744 は論文での使用が確認できていません。
