AB83917

Recombinant human CD40 protein (Fc Chimera Active)

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Recombinant human CD40 protein (Fc Chimera Active) is a Human Fragment protein, in the 21 to 193 aa range, expressed in HEK 293 cells, with >95%, suitable for SDS-PAGE, WB, FuncS.

別名を表示する

CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40

3 Images

Functional Studies - Recombinant human CD40 protein (Fc Chimera Active) (AB83917)

FuncS

Unknown

Functional Studies - Recombinant human CD40 protein (Fc Chimera Active) (AB83917)

Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.

Unknown

Western blot - Recombinant human CD40 protein (Fc Chimera Active) (AB83917)

All lanes:

Western blot - Anti-CD40 antibody (<a href='/products/primary-antibodies/cd40-antibody-ab13545'>ab13545</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 30 kDa

Observed band size: 72 kDa

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Exposure time: 8min

SDS-PAGE - Recombinant human CD40 protein (Fc Chimera Active) (AB83917)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CD40 protein (Fc Chimera Active) (AB83917)

1D SDS-PAGE of ab83917 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1 : ab83917
Lane 2 : ab83917 treated with PNGase F to remove potential N-linked glycans
Lane 3 : ab83917 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.

Key facts

精製度

>95% SDS-PAGE

発現系

HEK 293 cells

アプリケーション

FuncS, SDS-PAGE, WB

applications

生物活性

Yes

生物学的活性

Activity: The ED₅₀ of ab83917 is typically 1.0-1.5 ug/ml as measured by its ability to neutralize CD40 ligand mediated proliferation of T47-D cells.

アクセッション番号

P25942

アニマルフリー

キャリアフリー

種

Human

再構成

Reconstitute in 0.5 mL of PBS

バッファー組成

Constituents: PBS, 10% Trehalose, 1% Human serum albumin

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"pI: ab83917 separates into a number of glycoforms with an observed pI between 5.5 and 9.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified CD40- Fc Chimera that has a predicted pI of 6.0." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab83917 can be used as a WB positive control in conjunction with <a href='/products/primary-antibodies/cd40-antibody-ab13545'>ab13545</a>. Molecular Mass: Under reducing conditions ab83917 migrates as a broad band between 50 and 65 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified CD40-Fc Chimera that has a predicted monomeric molecular mass of 46.5 kDa." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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配列情報

[{"sequence":"EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDTWNRETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVLHRSCSPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLVVQQAGTNKTDVVCGPQDRLRRSSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":193,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P25942","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle|Reconstitute for long term storage

True

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Receptor for TNFSF5/CD40LG (PubMed : 31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information CD40

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Recombinant human CD40 protein (Fc Chimera Active)