Recombinant human CD28 protein (Active) (ab286013)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.200 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human CD28 protein (Active)
CD28 タンパク質・ペプチド 製品一覧 -
生理活性
Human biotinylated CD80, Fc Tag can bind to immobilized human CD28, Fc Tag at 1 µg/mL, 100 µL/well with a linear range of 0.03 – 0.81 µg/mL.
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精製度
>= 95 % SDS-PAGE. -
エンドトキシン・レベル
< 0.200 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
25 kDa -
分子量情報
66-70 kDa -
領域
19 to 152 -
タグ
Fc tag C-Terminus -
配列の追加情報
Source HEK 293 cells
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特性
Our Abpromise guarantee covers the use of ab286013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
備考
This product is manufactured by BioVision, an Abcam company and was previously called 9237 Human CellExp™ CD28, Human Recombinant. 9237-100 is the same size as the 100 µg size of ab286013.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute in sterile PBS, pH 7.4 to a concentration of 10 µg/ml
関連情報
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別名
- CD 28
- CD28
- CD28 antigen
see all -
機能
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
組織特異性
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
画像
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ELISA - Recombinant human CD28 protein (Active) (ab286013)
CD28 and CD80-Biotin ELISA binding activity:
Human biotinylated CD80, Fc Tag can bind to immobilized human CD28, Fc Tag at 1 µg/mL, 100 ng/well with a linear range of 0.03 – 0.81 µg/mL.
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SDS-PAGE - Recombinant human CD28 protein (Active) (ab286013)SDS-PAGE (4-20%): Human CD28, Fc-Tag protein was run on SDS PAGE gel under reducing (R) conditions and stained with Coomassie Blue.
Lane M: Protein Marker
Lane 1: Biotinylated Human CD28 Fc Tag (2 µg)
Lane 2: Human CD28 Fc Tag (2 µg).
The proteins migrate to around 55 kDa due to glycosylation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab286013 は論文での使用が確認できていません。
